ArticleActa pharmacologica Sinica2010
Carbachol inhibits TNF-α-induced endothelial barrier dysfunction through alpha 7 nicotinic receptors.
Article in Acta pharmacologica Sinica, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Optogenetic stimulation of basal forebrain cholinergic neurons prevents neuroinflammation and neuropsychiatric manifestations in pristane induced lupus mice.Behavioral and brain functions : BBF · 2023Article
- Carbachol, along with calcium, indicates new strategy in neural differentiation of human adipose tissue-derived mesenchymal stem cellsRegenerative therapy · 2023Article
- Cholinergic regulation of vascular endothelial function by human ChATProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cholinergic Activation of Primary Human Derived Intestinal Epithelium Does Not Ameliorate TNF-α Induced Injury.Cellular and molecular bioengineering · 2020Article
- Role of the α7 Nicotinic Acetylcholine Receptor in the Pathophysiology of Atherosclerosis.Frontiers in physiology · 2020Review
- miR-499 released during myocardial infarction causes endothelial injury by targeting α7-nAchR.Journal of cellular and molecular medicine · 2019Article
- Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in a canine burn model.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2017Article
- Xuebijing Injection () increases early survival rate by alleviating pulmonary vasopermeability in rats subjected to severe burns.Chinese journal of integrative medicine · 2017Article
- Electroacupuncturing at Zusanli point (ST36) attenuates pro-inflammatory cytokine release and organ dysfunction by activating cholinergic anti-inflammatory pathway in rat with endotoxin challenge.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2014Article
- Carbachol promotes gastrointestinal function during oral resuscitation of burn shock.World journal of gastroenterology · 2011Article
- Frontiers of vascular biology and disease research.Acta pharmacologica Sinica · 2010Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo test whether carbachol can influence endothelial barrier dysfunction induced by tumor necrosis factor (TNF)-α and whether the alpha 7 nicotinic receptor can mediate this process.
methodsRat cardiac microvascular endothelial cells were exposed to carbachol followed by TNF-α treatment in the presence or the absence of α-bungarotoxin (an antagonist of the alpha 7 nicotinic receptor). Permeability of endothelial cells cultured on Transwell filters was assayed using FITC-albumin. F-actin was stained with FITC- phalloidin. Expression of vascular endothelial cadherin, intercellular adhesion molecule 1 (ICAM-1), phosphor-ERK1/2 and phosphor-JNK was detected using Western blot.
resultsCarbachol (2 μmol/L-2 mmol/L) prevented increase in endothelial cell permeability induced by TNF-α (500 ng/mL) in a dose-dependent manner. Further, it attenuated the down-regulation of vascular endothelial cadherin and the up-regulation of ICAM-1 induced by TNF-α. In addition, treatment of endothelial cells with carbachol decreased phosphor-ERK1/2 and phosphor-JNK. These effects of carbachol were blocked by α-bungarotoxin 3 μg/mL.
conclusionThese data suggest that the inhibitory effect of carbachol on TNF-α-induced endothelial barrier dysfunction mediated by the alpha 7 nicotinic receptor.
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