ArticleProceedings of the National Academy of Sciences of the United States of America2010
Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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61 citing papers in PubMed, 94 citations in OpenAlex.
- Efficient production of cynomolgus monkeys via transabdominal uterine puncture embryo transfer†.Biology of reproduction · 2026Article
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- Liver-specific transgenic expression of human NTCP in rhesus macaques confers HBV susceptibility on primary hepatocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Developments in the study of Chinese herbal medicine's assessment index and action mechanism for diabetes mellitus.Animal models and experimental medicine · 2024Review
- Study of tree shrew biology and models: A booming and prosperous field for biomedical research.Zoological research · 2024Review
- Primate Model Carrying LMNA Mutation Develops Dilated Cardiomyopathy.JACC. Basic to translational science · 2024Article
- Rodent Models of Huntington's Disease: An Overview.Biomedicines · 2023Review
- Lineage-specific accelerated sequences underlying primate evolution.Science advances · 2023Article
- Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification.Journal of visualized experiments : JoVE · 2023Article
- Functional integration of Cas9 gene into the genome of rhesus monkey: possibility of a new biomedical model?Life medicine · 2023Article
- Cloning and base editing of GFP transgenic rhesus monkey and off-target analysis.Science advances · 2022Article
- Towards the peak: the 10-year journey of the National Research Facility for Phenotypic and Genetic Analysis of Model Animals (Primate Facility) and a call for international collaboration in non-human primate research.Zoological research · 2022Article
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- Modeling genetic diseases in nonhuman primates through embryonic and germline modification: Considerations and challenges.Science translational medicine · 2022Review
- Review
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- Gene editing monkeys: Retrospect and outlook.Frontiers in cell and developmental biology · 2022Review
- The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey.Cells · 2021Article
- A piggyBac-based platform for genome editing and clonal rhesus macaque iPSC line derivation.Scientific reports · 2021Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
22 authors at 9 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of transgenic technologies in monkeys is important for creating valuable animal models of human physiology so that the etiology of diseases can be studied and potential therapies for their amelioration may be developed. However, the efficiency of producing transgenic primate animals is presently very low, and there are few reports of success. We have developed an improved methodology for the production of transgenic rhesus monkeys, making use of a simian immunodeficiency virus (SIV)-based vector that encodes EGFP and a protocol for infection of early-cleavage-stage embryos. We show that infection does not alter embryo development. Moreover, the timing of infection, either before or during embryonic genome activation, has no observable effect on the level and stability of transgene expression. Of 70 embryos injected with concentrated virus at the one- to two-cell stage or the four- to eight-cell stage and showing fluorescence, 30 were transferred to surrogate mothers. One transgenic fetus was obtained from a fraternal triple pregnancy. Four infant monkeys were produced from four singleton pregnancies, of which two expressed EGFP throughout the whole body. These results demonstrate the usefulness of SIV-based lentiviral vectors for the generation of transgenic monkeys and improve the efficiency of transgenic technology in nonhuman primates.
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