ArticleEukaryotic cell2010
α-Tubulin mutations alter oryzalin affinity and microtubule assembly properties to confer dinitroaniline resistance.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Adaptations in Plasmodium tubulin determine distinct microtubule architectures, mechanics and drug susceptibility.Nature communications · 2026Article
- The Lipotubuloids ofPlants (Basel, Switzerland) · 2025Article
- The tubulin database: Linking mutations, modifications, ligands and local interactions.PloS one · 2023Article
- Unveiling the Possible Oryzalin-Binding Site in the α-Tubulin ofACS omega · 2022Article
- Identification of two Eleusine indica (goosegrass) biotypes of cool-season turfgrass resistant to dithiopyr.Pest management science · 2022Article
- The microtubule cytoskeleton: An old validated target for novel therapeutic drugs.Frontiers in pharmacology · 2022Review
- Systematic Analysis of Clemastine, a Candidate Apicomplexan Parasite-Selective Tubulin-Targeting Agent.International journal of molecular sciences · 2021Article
- Inhibiting parasite proliferation using a rationally designed anti-tubulin agent.EMBO molecular medicine · 2021Article
- Giardia duodenalis Virulence - "To Be, or Not To Be".Current tropical medicine reports · 2021Review
- Developing a novel artificial rice germplasm for dinitroaniline herbicide resistance by base editing of OsTubA2.Plant biotechnology journal · 2021Article
- Dinitroaniline Herbicide Resistance and Mechanisms in Weeds.Frontiers in plant science · 2021Review
- Pyridachlometyl has a novel anti-tubulin mode of action which could be useful in anti-resistance management.Pest management science · 2020Article
- Microtubule number and length determine cellular shape and function in Plasmodium.The EMBO journal · 2019Article
- Novel Peptide-Based Inhibitors for Microtubule Polymerization inInternational journal of molecular sciences · 2019Article
- Specific Targeting of Plant and Apicomplexa Parasite Tubulin through Differential Screening Using In Silico and Assay-Based Approaches.International journal of molecular sciences · 2018Article
- Novel α-Tubulin Mutations Conferring Resistance to Dinitroaniline Herbicides inFrontiers in plant science · 2018Article
- Microtubules in plants.The arabidopsis book · 2015Article
- Targeting Toxoplasma tubules: tubulin, microtubules, and associated proteins in a human pathogen.Eukaryotic cell · 2015Review
- Microtubule drugs: action, selectivity, and resistance across the kingdoms of life.Protoplasma · 2014Review
- Salt stress-induced disassembly of Arabidopsis cortical microtubule arrays involves 26S proteasome-dependent degradation of SPIRAL1.The Plant cell · 2011Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Plant and protozoan microtubules are selectively sensitive to dinitroanilines, which do not disrupt vertebrate or fungal microtubules. Tetrahymena thermophila is an abundant source of dinitroaniline-sensitive tubulin, and we have modified the single T. thermophila α-tubulin gene to create strains that solely express mutant α-tubulin in functional dimers. Previous research identified multiple α-tubulin mutations that confer dinitroaniline resistance in the human parasite Toxoplasma gondii, and when two of these mutations (L136F and I252L) were introduced into T. thermophila, they conferred resistance in these free-living ciliates. Purified tubulin heterodimers composed of L136F or I252L α-tubulin display decreased affinity for the dinitroaniline oryzalin relative to wild-type T. thermophila tubulin. Moreover, the L136F substitution dramatically reduces the critical concentration for microtubule assembly relative to the properties of wild-type T. thermophila tubulin. Our data provide additional support for the proposed dinitroaniline binding site on α-tubulin and validate the use of T. thermophila for expression of genetically homogeneous populations of mutant tubulins for biochemical characterization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.