ArticleMethods in cell biology2010
Intracellular membrane traffic at high resolution.
Article in Methods in cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Correlative light-electron microscopy using small gold nanoparticles as single probes.Light, science & applications · 2023Article
- High-Pressure Freezing Followed by Freeze Substitution: An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Chromogranin B (CHGB) is dimorphic and responsible for dominant anion channels delivered to cell surface via regulated secretion.Frontiers in molecular neuroscience · 2023Article
- TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages.Scientific reports · 2021Article
- High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy: Toward a Quantitative Nanoparticle-Cell Analysis.Nano letters · 2019Article
- Multiple membrane extrusion sites drive megakaryocyte migration into bone marrow blood vessels.Life science alliance · 2018Article
- Inducing fluorescence of uranyl acetate as a dual-purpose contrast agent for correlative light-electron microscopy with nanometre precision.Scientific reports · 2017Article
- A mammalian nervous-system-specific plasma membrane proteasome complex that modulates neuronal function.Nature structural & molecular biology · 2017Article
- Direct Evidence of Lack of Colocalisation of Fluorescently Labelled Gold Labels Used in Correlative Light Electron Microscopy.Scientific reports · 2017Article
- Exosomes and exosomal miRNAs in cardiovascular protection and repair.Vascular pharmacology · 2015Review
- The complex ultrastructure of the endolysosomal system.Cold Spring Harbor perspectives in biology · 2014Review
- Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking.Protoplasma · 2014Article
- Lactose as a "Trojan horse" for quantum dot cell transport.Angewandte Chemie (International ed. in English) · 2014Article
- Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways.Cells · 2013Article
- Virus factories: biogenesis and structural design.Cellular microbiology · 2013Review
- Virus morphogenesis in the cell: methods and observations.Sub-cellular biochemistry · 2013Review
- Multilamellar structures and filament bundles are found on the cell surface during bunyavirus egress.PloS one · 2013Article
- MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway.Molecular biology of the cell · 2012Article
- Correlative light and electron microscopy (CLEM) as a tool to visualize microinjected molecules and their eukaryotic sub-cellular targets.Journal of visualized experiments : JoVE · 2012Article
- Sorting nexins provide diversity for retromer-dependent trafficking events.Nature cell biology · 2011Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Membrane traffic between organelles is essential for a multitude of processes that maintain cell homeostasis. Many steps in these tightly regulated trafficking pathways take place in microdomains on the membranes of organelles, which require analysis at nanometer resolution. Electron microscopy (EM) can visualize these processes in detail and is mainly responsible for our current view of morphology on the subcellular level. This review discusses how EM can be applied to solve many questions of intracellular membrane traffic, with a focus on the endosomal system. We describe the expansion of the technique from purely morphological analysis to cryo-immuno-EM, correlative light electron microscopy (CLEM), and 3D electron tomography. In this review we go into some technical details of these various techniques. Furthermore, we provide a full protocol for immunolabeling on Lowicryl sections of high-pressure frozen cells as well as a detailed description of a simple CLEM method that can be applied to answer many membrane trafficking questions. We believe that these EM-based techniques are important tools to expand our understanding of the molecular details of endosomal sorting and intracellular membrane traffic in general.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.