Evidence map›Paper›PMID 20869258›Full record

ReviewMicron (Oxford, England : 1993)2011

Preparation and high-resolution microscopy of gold cluster labeled nucleic acid conjugates and nanodevices.

Richard D Powell, James F Hainfeld

Abstract readReview
In one paragraph

Review in Micron (Oxford, England : 1993), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Site-specific covalent labeling of large RNAs with nanoparticles empowered by expanded genetic alphabet transcription.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Richard D PowellNanoprobes, Incorporated, 95 Horseblock Road, Unit 1, Yaphank, NY 11980, United States. rpowell@nanoprobes.com
James F Hainfeld

Funding

Gold Quenched Molecular BeaconsR44GM062100 · NIGMS · NANOPROBES, INC. · PI POWELL, RICHARD DENIS · 2002 to 2003
$1.0M
Serial Blockface SEM Labels for Assessing Nervous System PlasticityR43MH086994 · NIMH · NANOPROBES, INC. · PI POWELL, RICHARD DENIS · 2009 to 2010
$700k
NIGMS NIH HHS R44 GM062100NIMH NIH HHS R43 MH086994
6 · The paper itself

Abstract

Nanogold and undecagold are covalently linked gold cluster labels which enable the identification and localization of biological components with molecular precision and resolution. They can be prepared with different reactivities, which means they can be conjugated to a wide variety of molecules, including nucleic acids, at specific, unique sites. The location of these sites can be synthetically programmed in order to preserve the binding affinity of the conjugate and impart novel characteristics and useful functionality. Methods for the conjugation of undecagold and Nanogold to DNA and RNA are discussed, and applications of labeled conjugates to the high-resolution microscopic identification of binding sites and characterization of biological macromolecular assemblies are described. In addition to providing insights into their molecular structure and function, high-resolution microscopic methods also show how Nanogold and undecagold conjugates can be synthetically assembled, or self-assemble, into supramolecular materials to which the gold cluster labels impart useful functionality.

Indexed as

Microscopy, ElectronNanotechnologyStaining and LabelingGoldHumansMetal NanoparticlesNucleic AcidsGoldNucleic Acids

Identifiers

PMID20869258
PMCPMC3583355

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.