ArticleParasitology research2011
Cloning and expression of 21.1-kDa tegumental protein of Clonorchis sinensis and human antibody response to it as a trematode-nematode pan-specific serodiagnosis antigen.
Article in Parasitology research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- One-pot nanoflower-based sensitive colorimetric biosensor for multihost detection of zoonotic clonorchiasis.PLoS neglected tropical diseases · 2026Article
- Liver Flukes: Clonorchis and Opisthorchis.Advances in experimental medicine and biology · 2024Review
- Comparative Characterization of Four Calcium-Binding EF Hand Proteins fromThe Korean journal of parasitology · 2018Article
- Current Perspectives on Opisthorchiasis Control and Cholangiocarcinoma Detection in Southeast Asia.Frontiers in medicine · 2018Review
- Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target.International journal of molecular sciences · 2017Article
- Current status and perspectives of Clonorchis sinensis and clonorchiasis: epidemiology, pathogenesis, omics, prevention and control.Infectious diseases of poverty · 2016Review
- Expression, immunolocalization, and serological reactivity of a novel sphingomyelin phosphodiesterase-like protein, an excretory/secretory antigen from Clonorchis sinensis.Parasitology research · 2013Article
- Identification and characterization of a novel 21.6-kDa tegumental protein from Clonorchis sinensis.Parasitology research · 2012Article
- Biochemical characterization and differential expression of a 16.5-kilodalton tegument-associated antigen from the liver fluke Fasciola hepatica.Clinical and vaccine immunology : CVI · 2012Article
- Cloning, expression, and characterization of a novel Opisthorchis viverrini calcium-binding EF-hand protein.Parasitology international · 2012Article
- Sequences and gene organization of the mitochondrial genomes of the liver flukes Opisthorchis viverrini and Clonorchis sinensis (Trematoda).Parasitology research · 2012Article
- The Schistosoma mansoni tegumental-allergen-like (TAL) protein family: influence of developmental expression on human IgE responses.PLoS neglected tropical diseases · 2012Article
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Authors and funding
7 authors.
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Abstract
A complete cDNA encoding a 21.1-kDa tegumental protein (CsTP21.1) was recognized from Clonorchis sinensis adult full-length cDNA plasmid library by bioinformatics analysis. Recombinant CsTP21.1 was highly expressed in Escherichia coli, purified by affinity chromatography, and identified by Western blotting. Immunohistochemistry demonstrated that CsTP21.1 is localized in the tegument of the adult worm. The rCsTP21.1-specific IgG1, IgG2, and IgG4 subclasses could be detected in the sera of clonorchiasis patients by ELISA, but their sensitivity was much lower than that of total IgG. The sensitivity and specificity of IgG in 66 serum samples of clonorchiasis patients were 100% and 95.5%, and the sensitivity was independent of worm loads; the cross-reaction rates in 86, 24, and 31 serum samples from patients infected with Fasciola hepatica, Schistosoma japonicum, and nematode were 98.8%, 83.3%, 93.3%, respectively, whereas no cross-reactions with Toxoplasma gondii and sparganum. This study demonstrated that CsTP21.1 is a trematode-nematode pan-specific antigen that is valuable in the development of a universal immunodiagnostic kit for human trematode and nematode infections.
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