ArticlePloS one2010
Detection of Mycobacterium leprae DNA from archaeological skeletal remains in Japan using whole genome amplification and polymerase chain reaction.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Leprosy in skeletons from archaeological sites: A systematic review.PLoS neglected tropical diseases · 2025Pooled it
- Evolutionary history ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Genomic Characterization ofFrontiers in microbiology · 2020Article
- A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides.BMC microbiology · 2018Article
- Quantitative evaluation of maxillary bone deformation by computed tomography in patients with leprosy.PLoS neglected tropical diseases · 2018Article
- Molecular analysis of ancient caries.Proceedings. Biological sciences · 2014Article
- Mycobacterium leprae genomes from a British medieval leprosy hospital: towards understanding an ancient epidemic.BMC genomics · 2014Article
- Article
- Comprehensive analysis of prokaryotes in environmental water using DNA microarray analysis and whole genome amplification.Pathogens (Basel, Switzerland) · 2013Article
- Detection and strain typing of ancient Mycobacterium leprae from a medieval leprosy hospital.PloS one · 2013Article
- Mycobacterium leprae: genes, pseudogenes and genetic diversity.Future microbiology · 2011Review
- Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIdentification of pathogen DNA from archaeological human remains is a powerful tool in demonstrating that the infectious disease existed in the past. However, it is very difficult to detect trace amounts of DNA remnants attached to the human skeleton, especially from those buried in a humid atmosphere with a relatively high environmental temperature such as in Asia. METHODOLOGY/PRINCIPAL
findingsHere we demonstrate Mycobacterium leprae DNA from archaeological skeletal remains in Japan by polymerase chain reaction, DNA sequencing and single nucleotide polymorphism (SNP) analysis. In addition, we have established a highly sensitive method of detecting DNA using a combination of whole genome amplification and polymerase chain reaction, or WGA-PCR, which provides superior sensitivity and specificity in detecting DNA from trace amounts of skeletal materials. CONCLUSION/SIGNIFICANCE: We have detected M. leprae DNA in archaeological skeletal remains for the first time in the Far East. Its SNP genotype corresponded to type 1; the first detected case worldwide of ancient M. leprae DNA. We also developed a highly sensitive method to detect ancient DNA by utilizing whole genome amplification.
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