Evidence map›Paper›PMID 20864349›Full record

ReviewMicron (Oxford, England : 1993)2011

Preparation of DNA and nucleoprotein samples for AFM imaging.

Yuri L Lyubchenko

Abstract readReview
In one paragraph

Review in Micron (Oxford, England : 1993), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
5.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 90 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Dynamic Behavior of RNA Nanoparticles Analyzed by AFM on a Mica/Air Interface.Langmuir : the ACS journal of surfaces and colloids · 2018
    Article
  12. Article
  13. Article
  14. Article
  15. Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.Critical reviews in eukaryotic gene expression · 2016
    Review
  16. Article
  17. Chromatin imaging with time-lapse atomic force microscopy.Methods in molecular biology (Clifton, N.J.) · 2015
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Yuri L LyubchenkoDepartment of Pharmaceutical Sciences, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE 68198-6025, United States. ylyubchenko@unmc.edu
University of Nebraska Medical Center · US

Funding

LOCAL AND GLOBAL STRUCTURES OF SUPERCOILED DNAR01GM062235 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI LYUBCHENKO, YURI L · 2001 to 2007
$1.7M
NIGMS NIH HHS GM 62235NIGMS NIH HHS R01 GM062235
6 · The paper itself

Abstract

Sample preparation techniques allowing reliable and reproducible imaging of DNA with various structures, topologies and complexes with proteins are reviewed. The major emphasis is given to methods utilizing chemical functionalization of mica, enabling preparation of the surfaces with required characteristics. The methods are illustrated by examples of imaging of different DNA structures. Special attention is given to the possibility of AFM to image the dynamics of DNA at the nanoscale. The capabilities of time-lapse AFM in aqueous solutions are illustrated by imaging of dynamic processes as transitions of local alternative structures (transition of DNA between H and B forms). The application of AFM to studies of protein-DNA complexes is illustrated by a few examples of imaging site-specific complexes, as well as such systems as chromatin. The time-lapse AFM studies of protein-DNA complexes including very recent advances with the use of high-speed AFM are reviewed.

Indexed as

DNADNA, SuperhelicalMicroscopy, Atomic ForceNucleic Acid ConformationNucleoproteinsDNADNA, SuperhelicalNucleoproteins

Identifiers

PMID20864349
PMCPMC2997872
OpenAlexW2072317160

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.