ArticleJournal of biomechanics2011
Effects of shear stress cultivation on cell membrane disruption and intracellular calcium concentration in sonoporation of endothelial cells.
Article in Journal of biomechanics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 79 citations in OpenAlex.
- Focused ultrasound in modern medicine: bioengineering interfaces, molecular effects, and clinical breakthroughs.Frontiers in bioengineering and biotechnology · 2025Review
- Friction in soft biological systems and surface self-organization: the role of viscoelasticity.Biophysical reviews · 2024Review
- Immunomodulation of human T cells by microbubble-mediated focused ultrasound.Frontiers in immunology · 2024Article
- Efficacy optimization of low frequency microbubble-mediated sonoporation as a drug delivery platform to cancer cells.International journal of pharmaceutics: X · 2022Article
- Landscape of Cellular Bioeffects Triggered by Ultrasound-Induced Sonoporation.International journal of molecular sciences · 2022Review
- Ultrasonic Microbubble Cavitation Enhanced Tissue Permeability and Drug Diffusion in Solid Tumor Therapy.Pharmaceutics · 2022Review
- A 3D finite element model to study the cavitation induced stresses on blood-vessel wall during the ultrasound-only phase of photo-mediated ultrasound therapy.AIP advances · 2022Article
- Article
- Theranostic Microbubbles with Homogeneous Ligand Distribution for Higher Binding Efficacy.Pharmaceutics · 2022Article
- Mechanically Induced Cavitation in Biological Systems.Life (Basel, Switzerland) · 2021Review
- Cytosolic CaTheranostics · 2021Article
- Ultrasound Imaging of Microbubble Activity during Sonoporation Pulse Sequences.Ultrasound in medicine & biology · 2019Article
- Overcoming transporter-mediated multidrug resistance in cancer: failures and achievements of the last decades.Drug delivery and translational research · 2019Review
- Sonoporation-induced cell membrane permeabilization and cytoskeleton disassembly at varied acoustic and microbubble-cell parameters.Scientific reports · 2018Article
- Mechanistic Insight into Sonoporation with Ultrasound-Stimulated Polymer Microbubbles.Ultrasound in medicine & biology · 2017Article
- Biophysical insight into mechanisms of sonoporation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications.Biomicrofluidics · 2016Review
- Bioorthogonal Click Chemistry-Based Synthetic Cell Glue.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Characterization of Bioeffects on Endothelial Cells under Acoustic Droplet Vaporization.Ultrasound in medicine & biology · 2015Article
- Two-bubble acoustic tweezing cytometry for biomechanical probing and stimulation of cells.Biophysical journal · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Microbubble facilitated ultrasound (US) application can enhance intracellular delivery of drugs and genes in endothelial cells cultured in static condition by transiently disrupting the cell membrane, or sonoporation. However, endothelial cells in vivo that are constantly exposed to blood flow may exhibit different sonoporation characteristics. This study investigates the effects of shear stress cultivation on sonoporation of endothelial cells in terms of membrane disruption and changes in the intracellular calcium concentration ([Ca(2+)](i)). Sonoporation experiments were conducted using murine brain microvascular endothelial (bEnd.3) cells and human umbilical vein endothelial cells (HUVECs) cultured under static or shear stress (5 dyne/cm(2) for 5 days) condition in a microchannel environment. The cells were exposed to a short US tone burst (1.25 MHz, 8 μs duration, 0.24 MPa) in the presence of Definity™ microbubbles to facilitate sonoporation. Membrane disruption was assessed by propidium iodide (PI) and changes in [Ca(2+)](i) measured by fura-2AM. Results from this study show that shear stress cultivation significantly reduced the impact of ultrasound-driven microbubbles activities on endothelial cells. Cells cultured under shear stress condition exhibited much lower percentage with membrane disruption and changes in [Ca(2+)](i) compared to statically cultured cells. The maximum increases of PI uptake and [Ca(2+)](i) were also significantly lower in the shear stress cultured cells. In addition, the extent of [Ca(2+)](i) waves in shear cultured HUVECs was reduced compared to the statically cultured cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.