ArticleBMC genomics2010
SOLiD sequencing of four Vibrio vulnificus genomes enables comparative genomic analysis and identification of candidate clade-specific virulence genes.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- Biochemical and Virulence Characterization ofFrontiers in cellular and infection microbiology · 2021Article
- Characterization of temperature-dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus.MicrobiologyOpen · 2019Article
- A stealth adhesion factor contributes to Vibrio vulnificus pathogenicity: Flp pili play roles in host invasion, survival in the blood stream and resistance to complement activation.PLoS pathogens · 2019Article
- Draft Genome Sequences of 42 Environmental Vibrio vulnificus Strains Isolated from the Northern Gulf of Mexico.Microbiology resource announcements · 2019Article
- Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains.PloS one · 2019Article
- A case ofJMM case reports · 2017Article
- Molecular and Physical Factors That Influence Attachment of Vibrio vulnificus to Chitin.Applied and environmental microbiology · 2015Article
- Role of anaerobiosis in capsule production and biofilm formation in Vibrio vulnificus.Infection and immunity · 2015Article
- Draft Genome Sequences of Four Closely Linked Vibrio vulnificus Isolates from the Biotype 1 Environmental Genotype.Genome announcements · 2015Article
- Different dynamics of genome content shuffling among host-specificity groups of the symbiotic actinobacterium Frankia.BMC genomics · 2014Article
- Implications of chitin attachment for the environmental persistence and clinical nature of the human pathogen Vibrio vulnificus.Applied and environmental microbiology · 2014Article
- Comparative genomic analysis of clinical and environmental Vibrio vulnificus isolates revealed biotype 3 evolutionary relationships.Frontiers in microbiology · 2014Article
- Differential expression of a sodium-phosphate cotransporter among Vibrio vulnificus strains.Microbial ecology · 2014Article
- Genome-wide SNP-genotyping array to study the evolution of the human pathogen Vibrio vulnificus biotype 3.PloS one · 2014Article
- Genotypic diversity and virulence characteristics of clinical and environmental Vibrio vulnificus isolates from the Baltic Sea region.Applied and environmental microbiology · 2013Article
- The interactions of Vibrio vulnificus and the oyster Crassostrea virginica.Microbial ecology · 2013Review
- Insight into the evolution of Vibrio vulnificus biotype 3's genome.Frontiers in microbiology · 2013Article
- Molecular typing of environmental and clinical strains of Vibrio vulnificus isolated in the northeastern USA.PloS one · 2013Article
- Sialic acid catabolism and transport gene clusters are lineage specific in Vibrio vulnificus.Applied and environmental microbiology · 2012Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVibrio vulnificus is the leading cause of reported death from consumption of seafood in the United States. Despite several decades of research on molecular pathogenesis, much remains to be learned about the mechanisms of virulence of this opportunistic bacterial pathogen. The two complete and annotated genomic DNA sequences of V. vulnificus belong to strains of clade 2, which is the predominant clade among clinical strains. Clade 2 strains generally possess higher virulence potential in animal models of disease compared with clade 1, which predominates among environmental strains. SOLiD sequencing of four V. vulnificus strains representing different clades (1 and 2) and biotypes (1 and 2) was used for comparative genomic analysis.
resultsGreater than 4,100,000 bases were sequenced of each strain, yielding approximately 100-fold coverage for each of the four genomes. Although the read lengths of SOLiD genomic sequencing were only 35 nt, we were able to make significant conclusions about the unique and shared sequences among the genomes, including identification of single nucleotide polymorphisms. Comparative analysis of the newly sequenced genomes to the existing reference genomes enabled the identification of 3,459 core V. vulnificus genes shared among all six strains and 80 clade 2-specific genes. We identified 523,161 SNPs among the six genomes.
conclusionsWe were able to glean much information about the genomic content of each strain using next generation sequencing. Flp pili, GGDEF proteins, and genomic island XII were identified as possible virulence factors because of their presence in virulent sequenced strains. Genomic comparisons also point toward the involvement of sialic acid catabolism in pathogenesis.
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