ArticleBiomedical microdevices2011
Measurement of contractile forces generated by individual fibroblasts on self-standing fiber scaffolds.
Article in Biomedical microdevices, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Two-Photon Polymerization: Fundamentals, Materials, and Chemical Modification Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- A Chemomechanical Model for Nuclear Morphology and Stresses during Cell Transendothelial Migration.Biophysical journal · 2016Article
- Substrates with patterned extracellular matrix and subcellular stiffness gradients reveal local biomechanical responses.Advanced materials (Deerfield Beach, Fla.) · 2014Article
- Regulation of the basement membrane by epithelia generated forces.Physical biology · 2012Article
- Dynamics of Stress Fibers Turnover in Contractile Cells.Journal of engineering mechanics · 2012Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contractility of cells in wound site is important to understand pathological wound healing and develop therapeutic strategies. In particular, contractile force generated by cells is a basic element for designing artificial three-dimensional cell culture scaffolds. Direct assessment of deformation of three-dimensional structured materials has been used to calculate contractile forces by averaging total forces with respect to the cell population number. However, macroscopic methods have offered only lower bounds of contractility due to experimental assumptions and the large variance of the spatial and temporal cell response. In the present study, cell contractility was examined microscopically in order to measure contractile forces generated by individual cells on self-standing fiber scaffolds that were fabricated via femtosecond laser-induced two-photon polymerization. Experimental assumptions and calculation errors that arose in previous studies of macroscopic and microscopic contractile force measurements could be reduced by adopting a columnar buckling model on individual, standing fiber scaffolds. Via quantifying eccentric critical loads for the buckling of fibers with various diameters, contractile forces of single cells were calculated in the range between 30-116 nN. In the present study, a force magnitude of approximately 200 nN is suggested as upper bound of the contractile force exerted by single cells. In addition, contractile forces by multiple cells on a single fiber were calculated in the range between 241-709 nN.
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Registered trials
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