Evidence map›Paper›PMID 20862549›Full record

ArticleMolecular biology reports2011

Genetic variation and phylogenetic relationship between two species of yellow catfish, Horabagrus brachysoma and H. nigricollaris (Teleostei: Horabagridae) based on RAPD and microsatellite markers.

P M A Muneer, A Gopalakrishnan, Remya Shivanandan, V S Basheer, A G Ponniah

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

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5citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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5 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

P M A MuneerNational Bureau of Fish Genetic Resources (NBFGR) Cochin Unit, CMFRI Campus, Cochin, 682 018, Kerala, India. pmamuneer@gmail.com
A Gopalakrishnan
Remya Shivanandan
V S Basheer
A G Ponniah

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The two species of yellow catfish, Horabagrus brachysoma and H. nigricollaris are categorized as 'endangered' and 'critically endangered' respectively in their wild habitat. Proper knowledge of genetic structure and variability of these endangered species are highly essential for the management, conservation and improvement of fish stocks. Therefore, genetic variation and phylogenetic relationships between these species of yellow catfish sampled from Chalakkudy River in the hot spot of biodiversity-Western Ghats region, Kerala, India were analyzed by using Random amplified polymorphic DNA (RAPD) and microsatellite markers. 85 RAPD and five microsatellites loci were detected to analyze the genetic variation and phylogenetic relationships among these species. Out of 85 RAPD loci produced only 52.94% were polymorphic whereas in microsatellite, all 5 loci were polymorphic (100%). Species-specific RAPD bands were found in both species studied. In microsatellite, the number of alleles across the five loci ranged from 1 to 8. The observed heterozygosities in H. brachysoma and H. nigricollaris were 0.463 and 0.443, respectively. Here, both RAPD and microsatellite methods reported a low degree of gene diversity and lack of genetic heterogeneity in both species of Horabagrus which strongly emphasize the need of fishery management, conservation and rehabilitation of these species.

Indexed as

Endangered SpeciesGenetic VariationPhylogenyAnimalsCatfishesConservation of Natural ResourcesDNA PrimersGene FrequencyIndiaMicrosatellite RepeatsRandom Amplified Polymorphic DNA TechniqueSpecies SpecificityDNA Primers

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