ArticlePLoS pathogens2010
Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection.
Article in PLoS pathogens, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
92 citing papers in PubMed, 138 citations in OpenAlex.
- RggmBio · 2026Article
- Virulence diversity amongJournal of oral microbiology · 2026Review
- The Effect of Antibiotic and Nonantibiotic Drugs on Plasmid-Mediated Bacterial Conjugation.International journal of microbiology · 2026Article
- Inferring Staphylococcus aureus host species and cross-species transmission from a genome-based model.BMC genomics · 2025Article
- Pneumococcal extracellular vesicles mediate horizontal gene transfer via the transformation machinery.mSphere · 2024Article
- Identification and genetic engineering of pneumococcal capsule-like polysaccharides in commensal oral streptococci.Microbiology spectrum · 2024Article
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- Flagellar interference with plasmid uptake in biofilms: a joint experimental and modeling study.Applied and environmental microbiology · 2024Article
- Pneumococcal Extracellular Vesicles Mediate Horizontal Gene Transfer via the Transformation Machinery.bioRxiv : the preprint server for biology · 2023Article
- Variants of Streptococcus pneumoniae Serotype 14 from Papua New Guinea with the Potential to Be Mistyped and Escape Vaccine-Induced Protection.Microbiology spectrum · 2022Article
- Repeat sequences limit the effectiveness of lateral gene transfer and favored the evolution of meiotic sex in early eukaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The acquisition of clinically relevant amoxicillin resistance in Streptococcus pneumoniae requires ordered horizontal gene transfer of four loci.PLoS pathogens · 2022Article
- Common Adaptive Strategies Underlie Within-Host Evolution of Bacterial Pathogens.Molecular biology and evolution · 2021Article
- Intra-Species Interactions inFrontiers in cellular and infection microbiology · 2021Article
- Control of Streptococcal Infections: Is a Common Vaccine Target Achievable AgainstFrontiers in microbiology · 2021Review
- Beyond the pan-genome: current perspectives on the functional and practical outcomes of the distributed genome hypothesis.Biochemical Society transactions · 2020Review
- Genome expansion in early eukaryotes drove the transition from lateral gene transfer to meiotic sex.eLife · 2020Article
- Multiplex gene transfer by genetic transformation between isolated S. pneumoniae cells confined in microfluidic droplets.Integrative biology : quantitative biosciences from nano to macro · 2019Article
- DprA-Dependent Exit from the Competent State Regulates Multifaceted Streptococcus pneumoniae Virulence.Infection and immunity · 2019Article
- Article
32 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
Abstract
Although there is tremendous interest in understanding the evolutionary roles of horizontal gene transfer (HGT) processes that occur during chronic polyclonal infections, to date there have been few studies that directly address this topic. We have characterized multiple HGT events that most likely occurred during polyclonal infection among nasopharyngeal strains of Streptococcus pneumoniae recovered from a child suffering from chronic upper respiratory and middle-ear infections. Whole genome sequencing and comparative genomics were performed on six isolates collected during symptomatic episodes over a period of seven months. From these comparisons we determined that five of the isolates were genetically highly similar and likely represented a dominant lineage. We analyzed all genic and allelic differences among all six isolates and found that all differences tended to occur within contiguous genomic blocks, suggestive of strain evolution by homologous recombination. From these analyses we identified three strains (two of which were recovered on two different occasions) that appear to have been derived sequentially, one from the next, each by multiple recombination events. We also identified a fourth strain that contains many of the genomic segments that differentiate the three highly related strains from one another, and have hypothesized that this fourth strain may have served as a donor multiple times in the evolution of the dominant strain line. The variations among the parent, daughter, and grand-daughter recombinant strains collectively cover greater than seven percent of the genome and are grouped into 23 chromosomal clusters. While capturing in vivo HGT, these data support the distributed genome hypothesis and suggest that a single competence event in pneumococci can result in the replacement of DNA at multiple non-adjacent loci.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.