ArticlePloS one2010
RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 51 citations in OpenAlex.
- Epizootic Haemorrhagic Disease Virus (EHDV) Infection in Red Deer (Animals : an open access journal from MDPI · 2026Article
- Evidence of exposure to bluetongue and epizootic haemorrhagic disease viruses in the ruminant population of Mauritius and Rodrigues Islands.Tropical animal health and production · 2025Article
- Review
- The Molecular Epidemiology of Epizootic Hemorrhagic Disease Viruses Identified in Israel between 2015 and 2023.Epidemiologia (Basel, Switzerland) · 2024Article
- Virus Neutralization Test for Detecting and Quantifying Serum-Neutralizing Antibodies to Epizootic Hemorrhagic Disease Virus (EHDV) (Serotypes 1, 2, and 4-8).Methods in molecular biology (Clifton, N.J.) · 2024Article
- End-Point RT-PCR Assays for Detection and Typing of Epizootic Hemorrhagic Disease Virus.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A serologic investigation of epizootic hemorrhagic disease virus in China between 2014 and 2019.Virologica Sinica · 2022Article
- Development of a Competitive Enzyme-Linked Immunosorbent Assay Based on Purified Recombinant Viral Protein 7 for Serological Diagnosis of Epizootic Haemorrhagic Disease in Camels.Journal of tropical medicine · 2022Article
- Article
- Novel Serotype of Epizootic Hemorrhagic Disease Virus, China.Emerging infectious diseases · 2020Article
- Epizootic Hemorrhagic Disease Virus Serotype 6 Infection in Cattle, Japan, 2015.Emerging infectious diseases · 2018Article
- Analysis of Conserved, Computationally Predicted Epitope Regions for VP5 and VP7 Across three Orbiviruses.Bioinformatics and biology insights · 2018Article
- Development of Real-Time RT-PCR Assays for Detection and Typing of Epizootic Haemorrhagic Disease Virus.Transboundary and emerging diseases · 2017Article
- Monitoring for bovine arboviruses in the most southwestern islands in Japan between 1994 and 2014.BMC veterinary research · 2016Article
- Reemergence of Ibaraki disease in southern Japan in 2013.The Journal of veterinary medical science · 2015Article
- Duplex Real-Time RT-PCR Assays for the Detection and Typing of Epizootic Haemorrhagic Disease Virus.PloS one · 2015Article
- Bluetongue and epizootic haemorrhagic disease virus in local breeds of cattle in Kenya.Research in veterinary science · 2013Article
- Full genome sequencing of Corriparta virus, identifies California mosquito pool virus as a member of the Corriparta virus species.PloS one · 2013Article
- Vector competence of Culicoides sonorensis (Diptera: Ceratopogonidae) to epizootic hemorrhagic disease virus serotype 7.Parasites & vectors · 2012Article
- Rapid molecular detection methods for arboviruses of livestock of importance to northern Europe.Journal of biomedicine & biotechnology · 2012Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 4 countries.
Funding
Abstract
Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genome-segment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus "serotype", as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV "types". Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.