ArticleCellular and molecular bioengineering2010
Mechanical Forces in Endothelial Cells during Firm Adhesion and Early Transmigration of Human Monocytes.
Article in Cellular and molecular bioengineering, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Neutrophils exhibit distinct migration phenotypes that are modulated by transendothelial migration.Communications biology · 2026Article
- Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications.Small science · 2025Review
- Cancer cells impact the microrheology of endothelial cells during physical contact or through paracrine signalling.Scientific reports · 2025Article
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Mechanotransduction in Endothelial Cells in Vicinity of Cancer Cells.Cellular and molecular bioengineering · 2022Article
- Article
- Soft Polymer-Based Technique for Cellular Force Sensing.Polymers · 2021Review
- Shear forces induce ICAM-1 nanoclustering on endothelial cells that impact on T-cell migration.Biophysical journal · 2021Article
- PECAM-1 supports leukocyte diapedesis by tension-dependent dephosphorylation of VE-cadherin.The EMBO journal · 2021Article
- Review
- Elucidating the Biomechanics of Leukocyte Transendothelial Migration by Quantitative Imaging.Frontiers in cell and developmental biology · 2021Review
- (De)form and Function: Measuring Cellular Forces with Deformable Materials and Deformable Structures.Advanced healthcare materials · 2020Review
- The biophysics and mechanics of blood from a materials perspective.Nature reviews. Materials · 2019Article
- Three-dimensional forces exerted by leukocytes and vascular endothelial cells dynamically facilitate diapedesis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Using Nesprin Tension Sensors to Measure Force on the LINC Complex.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Membrane-bound ICAM-1 contributes to the onset of proinvasive tumor stroma by controlling acto-myosin contractility in carcinoma-associated fibroblasts.Oncotarget · 2017Article
- Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF.Molecular biology of the cell · 2016Article
- Nesprin-2G, a Component of the Nuclear LINC Complex, Is Subject to Myosin-Dependent Tension.Biophysical journal · 2016Article
- Regulation of RhoA activity by adhesion molecules and mechanotransduction.Current molecular medicine · 2014Review
- Rho GAPs and GEFs: controling switches in endothelial cell adhesion.Cell adhesion & migration · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Transmigration of leukocytes across the endothelial barrier is a tightly controlled process involving multiple steps, including rolling adhesion, firm adhesion, and then penetration of leukocytes through the endothelial monolayer. While the key molecular signals have been described in great detail, we are only just beginning to unveil the mechanical forces involved in this process. Here, using a microfabricated system that reports traction forces generated by cells, we describe forces generated by endothelial cells during monocyte firm adhesion and transmigration. Average traction force across the endothelial monolayer increased dramatically when monocytes firmly adhered and transmigrated. Interestingly, the endothelial cell that was in direct contact with the monocyte exhibited much larger traction forces relative to its neighbors, and the direction of these traction forces aligned centripetally with respect to the monocyte. The increase in traction force occurred in the local subcellular zone of monocyte adhesion, and dissipated rapidly with distance. To begin to characterize the basis for this mechanical effect, we show that beads coated with anti-ICAM-1 or VCAM-1 antibodies bound to monolayers could reproduce this effect. Taken together, this study provides a new approach to examining the role of cellular mechanics in regulating leukocyte transmigration through the endothelium.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.