ArticleJournal of genetics2010
SSR mining in oil palm EST database: application in oil palm germplasm diversity studies.
Article in Journal of genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Unveiling the Secrets of Oil Palm Genetics: A Look into Omics Research.International journal of molecular sciences · 2024Review
- Inflorescence Transcriptome Sequencing and Development of New EST-SSR Markers in Common Buckwheat (Plants (Basel, Switzerland) · 2022Article
- Phenomics, genomics of oil palm (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Genetic Diversity Assessment of MPOB-Senegal Oil Palm Germplasm Using Microsatellite Markers.BioMed research international · 2021Article
- Article
- Development of EST-SSR markers for genetic diversity analysis in coconut (Cocos nucifera L.).Molecular biology reports · 2020Article
- Article
- Identification of QTLs in oil palm (Journal of genetics · 2020Article
- SSR identification and marker development for sago palm based on NGS genome data.Breeding science · 2019Article
- Development and validation of whole genome-wide and genic microsatellite markers in oil palm (Elaeis guineensis Jacq.): First microsatellite database (OpSatdb).Scientific reports · 2019Article
- Application of Proteomics Technologies in Oil Palm Research.The protein journal · 2018Review
- Development of novel EST-SSR markers in the macaúba palm (Acrocomia aculeata) using transcriptome sequencing and cross-species transferability in Arecaceae species.BMC plant biology · 2018Article
- Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm.Scientific reports · 2017Article
- In Silico Approach for Characterization and Comparison of Repeats in the Genomes of Oil and Date Palms.Bioinformatics and biology insights · 2017Article
- Systemic Identification ofJournal of nucleic acids · 2017Article
- De novo assembly and functional annotation of Myrciaria dubia fruit transcriptome reveals multiple metabolic pathways for L-ascorbic acid biosynthesis.BMC genomics · 2015Article
- De novo assembly of transcriptome sequencing in Caragana korshinskii Kom. and characterization of EST-SSR markers.PloS one · 2015Article
- Exploiting transcriptome data for the development and characterization of gene-based SSR markers related to cold tolerance in oil palm (Elaeis guineensis).BMC plant biology · 2014Article
- Analyses of hypomethylated oil palm gene space.PloS one · 2014Article
- Identification of QTLs associated with callogenesis and embryogenesis in oil palm using genetic linkage maps improved with SSR markers.PloS one · 2013Article
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8 authors.
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Abstract
This study reports on the detection of additional expressed sequence tags (EST) derived simple sequence repeat (SSR) markers for the oil palm. A large collection of 19243 Elaeis guineensis ESTs were assembled to give 10258 unique sequences, of which 629 ESTs were found to contain 722 SSRs with a variety of motifs. Dinucleotide repeats formed the largest group (45.6%) consisting of 66.9% AG/CT, 21.9% AT/AT, 10.9% AC/GT and 0.3% CG/CG motifs. This was followed by trinucleotide repeats, which is the second most abundant repeat types (34.5%) consisting of AAG/CTT (23.3%), AGG/CCT (13.7%), CCG/CGG (11.2%), AAT/ATT (10.8%), AGC/GCT (10.0%), ACT/AGT (8.8%), ACG/CGT (7.6%), ACC/GGT (7.2%), AAC/GTT (3.6%) and AGT/ACT (3.6%) motifs. Primer pairs were designed for 405 unique EST-SSRs and 15 of these were used to genotype 105 E. guineensis and 30 E. oleifera accessions. Fourteen SSRs were polymorphic in at least one germplasm revealing a total of 101 alleles. The high percentage (78.0%) of alleles found to be specific for either E. guineensis or E. oleifera has increased the power for discriminating the two species. The estimates of genetic differentiation detected by EST-SSRs were compared to those reported previously. The transferability across palm taxa to two Cocos nucifera and six exotic palms is also presented. The polymerase chain reaction (PCR) products of three primer-pairs detected in E. guineensis, E. oleifera, C. nucifera and Jessinia bataua were cloned and sequenced. Sequence alignments showed mutations within the SSR site and the flanking regions. Phenetic analysis based on the sequence data revealed that C. nucifera is closer to oil palm compared to J. bataua; consistent with the taxanomic classification.
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