ArticleInvestigative ophthalmology & visual science2011
Properties of connexin 46 hemichannels in dissociated lens fiber cells.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 45 citations in OpenAlex.
- Mechano-activated connexin hemichannels and glutathione transport protect lens fiber cells against oxidative insults.Redox biology · 2024Article
- Connexins in Cancer, the Possible Role of Connexin46 as a Cancer Stem Cell-Determining Protein.Biomolecules · 2023Review
- Protein kinase A activation alleviates cataract formation via increased gap junction intercellular communication.iScience · 2023Article
- Patch clamp studies on TRPV4-dependent hemichannel activation in lens epithelium.Frontiers in pharmacology · 2023Article
- Mechanical Stress Modulates Calcium-Activated-Chloride Currents in Differentiating Lens Cells.Frontiers in physiology · 2022Article
- Hyposmotic stress causes ATP release in a discrete zone within the outer cortex of rat lens.Molecular vision · 2022Article
- Role and Posttranslational Regulation of Cx46 Hemichannels and Gap Junction Channels in the Eye Lens.Frontiers in physiology · 2022Review
- Mechanosensitive collaboration between integrins and connexins allows nutrient and antioxidant transport into the lens.The Journal of cell biology · 2020Article
- Regulation of Connexin Gap Junctions and Hemichannels by Calcium and Calcium Binding Protein Calmodulin.International journal of molecular sciences · 2020Review
- Connexin-46 Contained in Extracellular Vesicles Enhance Malignancy Features in Breast Cancer Cells.Biomolecules · 2020Article
- Calcium-Activated Chloride Channels in Newly Differentiating Mouse Lens Fiber Cells and Their Role in Volume Regulation.Investigative ophthalmology & visual science · 2019Article
- Concatenation of Human Connexin26 (hCx26) and Human Connexin46 (hCx46) for the Analysis of Heteromeric Gap Junction Hemichannels and Heterotypic Gap Junction Channels.International journal of molecular sciences · 2018Article
- Whole-genome sequencing reveals a recurrent missense mutation in the Connexin 46 (GJA3) gene causing autosomal-dominant lamellar cataract.Eye (London, England) · 2018Article
- Connexin hemichannels mediate glutathione transport and protect lens fiber cells from oxidative stress.Journal of cell science · 2018Article
- Cataract-associated connexin 46 mutation alters its interaction with calmodulin and function of hemichannels.The Journal of biological chemistry · 2018Article
- Focus on lens connexins.BMC cell biology · 2017Review
- A novel frameshift mutation in CX46 associated with hereditary dominant cataracts in a Chinese family.International journal of ophthalmology · 2017Article
- Connexin23 deletion does not affect lens transparency.Experimental eye research · 2016Article
- The connexin46 mutant, Cx46T19M, causes loss of gap junction function and alters hemi-channel gating.The Journal of membrane biology · 2015Article
- Neuron-Glia Crosstalk in the Autonomic Nervous System and Its Possible Role in the Progression of Metabolic Syndrome: A New Hypothesis.Frontiers in physiology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeTo characterize the properties of connexin 46 hemichannels in differentiating fiber cells isolated from mouse lenses.
methodsDifferentiating fiber cells were isolated from mouse lenses using collagenase. Cellular localization of connexin 50 (Cx50) and connexin 46 (Cx46) was assessed by immunofluorescence. Membrane currents were recorded using whole cell patch clamping. Dye uptake was measured using time-lapse imaging.
resultsIn freshly dissociated fiber cells isolated from knockout Cx50 (KOCx50) mouse lenses, removal of external divalent cations induced a macroscopic current composed of large conductance channels. This current was reduced at a holding potential of -60 mV, activated on depolarization, and had a reversal potential near 0 mV. These properties were very similar to those of Cx46 hemichannel currents recorded in single Xenopus oocytes. If the currents observed in divalent cation-free Ringer's solution were due to Cx46 hemichannel opening, then dye influx by gap junctional/hemichannel permeable dyes should be measurable in the fiber cells. To measure dye influx, the authors used the positively charged dyes, propidium iodide (PrI) and 4'-6-diamidino-2-phenylindole (DAPI). In the absence of external calcium, fiber cells took up both dyes. Furthermore, dye influx could be inhibited by hemichannel blockers. To confirm that this current was due to Cx46 hemichannels, the authors studied fiber cells isolated from the lenses of double knockout (Cx46(-/-); Cx50(-/-)) mice and demonstrated that both the calcium-sensitive conductance and dye influx were absent.
conclusionsThese results show that Cx46 can form functional hemichannels in the nonjunctional membrane of fiber cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.