ArticleInvestigative ophthalmology & visual science2011
RhoA inactivation prevents photoreceptor axon retraction in an in vitro model of acute retinal detachment.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- RPE With ROCK-Mediated Epithelial-Mesenchymal Transition as a Key Contributor of Subretinal Fibrosis in AMD.Investigative ophthalmology & visual science · 2026Article
- Synaptopathy in cone and rod cells after retinal detachment and reattachment.Experimental eye research · 2026Article
- Qualitative evaluations of reactive microglial heterogeneity in cultured porcine retina.Histology and histopathology · 2024Article
- Unveiling the role of CaMKII in retinal degeneration: from biological mechanism to therapeutic strategies.Cell & bioscience · 2024Review
- Article
- Microglia in Cultured Porcine Retina: Qualitative Immunohistochemical Analyses of Reactive Microglia in the Outer Retina.International journal of molecular sciences · 2023Article
- Human photoreceptors switch from autonomous axon extension to cell-mediated process pulling during synaptic marker redistribution.Cell reports · 2022Article
- Single-cell RNA sequencing of the retina in a model of retinitis pigmentosa reveals early responses to degeneration in rods and cones.BMC biology · 2022Article
- Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow.PloS one · 2022Article
- Coming of Age for the Photoreceptor Synapse.Investigative ophthalmology & visual science · 2021Article
- ROCK inhibition reduces morphological and functional damage to rod synapses after retinal injury.Scientific reports · 2021Article
- Critical Decrease in the Level of Axon Guidance Receptor ROBO1 in Rod Synaptic Terminals Is Followed by Axon Retraction.Investigative ophthalmology & visual science · 2020Article
- Rescue of Rod Synapses by Induction of Cav Alpha 1F in the Mature Cav1.4 Knock-Out Mouse Retina.Investigative ophthalmology & visual science · 2019Article
- Rho kinase inhibitors-a review on the physiology and clinical use in Ophthalmology.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Review
- Actin Dynamics, Regulated by RhoA-LIMK-Cofilin Signaling, Mediates Rod Photoreceptor Axonal Retraction After Retinal Injury.Investigative ophthalmology & visual science · 2019Article
- Concise Review: Update on Retinal Pigment Epithelium Transplantation for Age-Related Macular Degeneration.Stem cells translational medicine · 2019Review
- Calsenilin, a Presenilin Interactor, Regulates RhoA Signaling and Neurite Outgrowth.International journal of molecular sciences · 2018Article
- Sema3A Reduces Sprouting of Adult Rod Photoreceptors In Vitro.Investigative ophthalmology & visual science · 2017Article
- Fasudil, a Clinically Used ROCK Inhibitor, Stabilizes Rod Photoreceptor Synapses after Retinal Detachment.Translational vision science & technology · 2017Article
- The role of RhoA in retrograde neuronal death and axon regeneration after spinal cord injury.Neurobiology of disease · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeAn early injury response to retinal detachment is disruption of synaptic connectivity between photoreceptors and second-order neurons. Most dramatic is the retraction of rod cell axons and their terminals away from the outer synaptic layer and toward their cell bodies. This study tested whether axonal retraction in detached retina was due to the activation of the small GTPase RhoA and was preventable using RhoA antagonists.
methodsRetinal detachments were created in in vitro preparations of porcine eyecups. RhoA activation was determined with a Rhotekin binding assay. To block axon retraction, drugs were applied to neural retinal explants either before or after detachment from the retinal pigment epithelium. Presynaptic movement was quantified by image analysis of double-labeled retinas examined with confocal microscopy.
resultsActive RhoA increases transiently after detachment followed by morphologic evidence of axonal retraction over the next 24 hours. Pretreating the retina with a RhoA antagonist, CT-04, or a Rho kinase inhibitor, Y27632, at multiple concentrations significantly inhibited axonal retraction. Reducing calcium influx through L-type calcium channels with nicardipine also blocked retraction. To create a more plausible therapeutic scenario, drug treatments were delayed and applied after retinal detachment. The Rho kinase inhibitor, but not nicardipine, significantly blocked rod axonal retraction when applied up to 6 hours after detachment.
conclusionsThus, RhoA and downstream Rho kinase activity constitute part of the mechanism that produces rod axonal retraction in retinal explants. Treatments that manipulate RhoA signaling may promote synaptic stability after retinal detachment.
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