ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2010
Estradiol rapidly regulates membrane estrogen receptor alpha levels in hypothalamic neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 100 citations in OpenAlex.
- Estrogen Influences Human Microvascular Endothelial Function Via Sex-Specific Regulation of Sphingolipids.JACC. Basic to translational science · 2026Article
- Microencapsulated rrBNGF as an alternative ovulation induction method in rabbits.BMC veterinary research · 2025Article
- Localisation of oestrogen receptors in stem cells and in stem cell-derived neurons of the mouse.Journal of neuroendocrinology · 2023Article
- Endolysosome Localization of ERα Is Involved in the Protective Effect of 17α-Estradiol against HIV-1 gp120-Induced Neuronal Injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Estrogen Receptors as Molecular Targets of Endocrine Therapy for Glioblastoma.International journal of molecular sciences · 2021Review
- Impact of estrogen receptor agonists and model of menopause on enzymes involved in brain metabolism, acetyl-CoA production and cholinergic function.Life sciences · 2020Article
- ERαΔ4, an ERα splice variant missing exon4, interacts with caveolin-3 and mGluR2/3.Journal of neuroendocrinology · 2019Article
- A link between plasma membrane calcium ATPase 2 (PMCA2), estrogen and estrogen receptor α signaling in mechanical pain.Scientific reports · 2018Article
- Extranuclear signaling by ovarian steroids in the regulation of sexual receptivity.Hormones and behavior · 2018Review
- Estrogen and Progesterone Integration in an in vitro Model of RP3V Kisspeptin Neurons.Neuroendocrinology · 2018Article
- Estradiol Membrane-Initiated Signaling in the Brain Mediates Reproduction.Trends in neurosciences · 2017Review
- Update on the therapeutic significance of estrogen receptor beta in malignant gliomas.Oncotarget · 2017Review
- Cooperation of Genomic and Rapid Nongenomic Actions of Estrogens in Synaptic Plasticity.Molecular neurobiology · 2017Review
- Integrating Neural Circuits Controlling Female Sexual Behavior.Frontiers in systems neuroscience · 2017Review
- CNTF-Treated Astrocyte Conditioned Medium Enhances Large-Conductance Calcium-Activated Potassium Channel Activity in Rat Cortical Neurons.Neurochemical research · 2016Article
- Hippocampal Fast Glutamatergic Transmission Is Transiently Regulated by Corticosterone Pulsatility.PloS one · 2016Article
- Rapid Effects of Estradiol on Aggression in Birds and Mice: The Fast and the Furious.Integrative and comparative biology · 2015Review
- Estradiol Membrane-Initiated Signaling and Female Reproduction.Comprehensive Physiology · 2015Review
- Rapid modulation of synaptogenesis and spinogenesis by 17β-estradiol in primary cortical neurons.Frontiers in cellular neuroscience · 2015Article
- Regulation of Estrogen Receptor α Expression in the Hypothalamus by Sex Steroids: Implication in the Regulation of Energy Homeostasis.International journal of endocrinology · 2015Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Estrogen receptors (ERs) and estrogen-binding proteins have been localized intracellularly and on the cell surface. The membrane-associated proteins initiate signaling that activates a myriad of cellular responses including the modulation of ion channels and ultimately transcription. Although many of the downstream actions of membrane ERs, including ERα and ERβ, have been characterized, the mechanisms regulating membrane ER levels have remained elusive in the nervous system. In the present study, we used surface biotinylation to identify and study the estradiol regulation of membrane ERα in mixed-sex, cultured hypothalamic neurons from rat. Following surface biotinylation, Western blot analysis revealed full-length 66 kDa ERα and several ERα splice variants, most notably a biotinylated 52 kDa ERα-immunoreactive protein. Treatment of the neurons with estradiol caused a rapid and transient increase of the biotinylated 52 kDa and 66 kDa ERα proteins in the plasma membrane. Exposure of the neurons to estradiol also significantly increased internalization of 52 kDa and 66 kDa ERα membrane proteins, a measure of receptor activation. In the hypothalamus, membrane ERα signaling depends on transactivation of metabotropic glutamate receptor-1a (mGluR1a). Estradiol treatment increased the internalization of mGluR1a in parallel with ERα, a finding consistent with the hypothesis of an ERα-mGluR1a signaling unit. These results demonstrate that estradiol regulates the amount of ERα in the membrane, suggesting estradiol can regulate its own membrane signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.