ArticleMolecular biology of the cell2010
Jen1p: a high affinity selenite transporter in yeast.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 84 citations in OpenAlex.
- Biofortification of Yeast with Selenomethionine in Corn Hydrolysate: Influence of Different Metabolic Pathways.Biological trace element research · 2026Article
- A genetic screen reveals a key role for Reg1 in 2-deoxyglucose sensing and yeast AMPK inhibition.PLoS genetics · 2025Article
- Biodegradation of sodium selenite by a highly tolerant strainCurrent research in microbial sciences · 2025Article
- Characterization and biological activity of selenium nanoparticles biosynthesized by Yarrowia lipolytica.Microbial biotechnology · 2024Article
- Article
- Mechanistic characterization of waterborne selenite uptake in the water flea,Conservation physiology · 2024Article
- Optimization of Se- and Zn-Enriched Mycelium ofNutrients · 2023Article
- High-Throughput Profiling of Candida auris Isolates Reveals Clade-Specific Metabolic Differences.Microbiology spectrum · 2023Review
- Adaptive Laboratory Evolution Reveals the Selenium Efflux Process To Improve Selenium Tolerance Mediated by the Membrane Sulfite Pump in Saccharomyces cerevisiae.Microbiology spectrum · 2023Article
- Engineering Flocculation for Improved Tolerance and Production of d-Lactic Acid inJournal of fungi (Basel, Switzerland) · 2023Article
- Mechanisms Affecting the Biosynthesis and Incorporation Rate of Selenocysteine.Molecules (Basel, Switzerland) · 2021Review
- Article
- Mechanisms underlying lactic acid tolerance and its influence on lactic acid production inMicrobial cell (Graz, Austria) · 2021Review
- Endocytosis of nutrient transporters in fungi: The ART of connecting signaling and trafficking.Computational and structural biotechnology journal · 2021Review
- Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications.FEMS microbiology letters · 2020Review
- Metabolic Response of the YeastInternational journal of molecular sciences · 2020Article
- Carboxylic Acid Transporters inmBio · 2020Review
- Selenium Biofortification of Crop Food by Beneficial Microorganisms.Journal of fungi (Basel, Switzerland) · 2020Review
- A cysteinyl-tRNA synthetase variant confers resistance against selenite toxicity and decreases selenocysteine misincorporation.The Journal of biological chemistry · 2019Article
- Directed Evolution ofMicroorganisms · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Selenium is a micronutrient in most eukaryotes, including humans, which is well known for having an extremely thin border between beneficial and toxic concentrations. Soluble tetravalent selenite is the predominant environmental form and also the form that is applied in the treatment of human diseases. To acquire this nutrient from low environmental concentrations as well as to avoid toxicity, a well-controlled transport system is required. Here we report that Jen1p, a proton-coupled monocarboxylate transporter in S. cerevisiae, catalyzes high-affinity uptake of selenite. Disruption of JEN1 resulted in selenite resistance, and overexpression resulted in selenite hypersensitivity. Transport assay showed that overexpression of Jen1p enables selenite accumulation in yeast compared with a JEN1 knock out strain, indicating the Jen1p transporter facilitates selenite accumulation inside cells. Selenite uptake by Jen1p had a Km of 0.91 mM, which is comparable to the Km for lactate. Jen1p transported selenite in a proton-dependent manner which resembles the transport mechanism for lactate. In addition, selenite and lactate can inhibit the transport of each other competitively. Therefore, we postulate selenite is a molecular mimic of monocarboxylates which allows selenite to be transported by Jen1p.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.