ArticleEndocrinology2010
Epigenetic changes coincide with in vitro primate GnRH neuronal maturation.
Article in Endocrinology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Methylome analysis in girls with idiopathic central precocious puberty.Clinical epigenetics · 2024Article
- The mechanism underlying the pubertal increase in pulsatile GnRH release in primates.Journal of neuroendocrinology · 2022Review
- Genetic and Epigenetic Control of Puberty.Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation · 2022Review
- Methylation status of hypothalamicFrontiers in endocrinology · 2022Article
- MKRN3 role in regulating pubertal onset: the state of art of functional studies.Frontiers in endocrinology · 2022Review
- Emerging Roles of Epigenetics in the Control of Reproductive Function: Focus on Central Neuroendocrine Mechanisms.Journal of the Endocrine Society · 2021Review
- Epigenetics of the developing and aging brain: Mechanisms that regulate onset and outcomes of brain reorganization.Neuroscience and biobehavioral reviews · 2021Review
- Unravelling the role of epigenetics in reproductive adaptations to early-life environment.Nature reviews. Endocrinology · 2020Review
- Mechanism of pulsatile GnRH release in primates: Unresolved questions.Molecular and cellular endocrinology · 2019Review
- Minireview: The Epigenetic Modulation ofInternational journal of environmental research and public health · 2019Review
- Molecular and Environmental Mechanisms Regulating Puberty Initiation: An Integrated Approach.Frontiers in endocrinology · 2019Review
- The Emerging Role of Chromatin Remodeling Factors in Female Pubertal Development.Neuroendocrinology · 2019Review
- Hypothalamic epigenetics driving female puberty.Journal of neuroendocrinology · 2018Review
- The Methylcytosine Dioxygenase Ten-Eleven Translocase-2 (tet2) Enables Elevated GnRH Gene Expression and Maintenance of Male Reproductive Function.Endocrinology · 2016Article
- Kisspeptin Induces Dynamic Chromatin Modifications to Control GnRH Gene Expression.Molecular neurobiology · 2016Article
- Article
- The Emerging Role of Epigenetics in the Regulation of Female Puberty.Endocrine development · 2016Review
- Fibroblast Growth Factor 8 Expression in GT1-7 GnRH-Secreting Neurons Is Androgen-Independent, but Can Be Upregulated by the Inhibition of DNA Methyltransferases.Frontiers in cell and developmental biology · 2016Article
- Epigenetic regulation of female puberty.Frontiers in neuroendocrinology · 2015Review
- A system biology approach to identify regulatory pathways underlying the neuroendocrine control of female puberty in rats and nonhuman primates.Hormones and behavior · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cellular and molecular mechanisms underlying pulsatile GnRH release are not well understood. In the present study, we examined the developmental changes in intracellular calcium dynamics, peptide release, gene expression, and DNA methylation in cultured GnRH neurons derived from the nasal placode of rhesus monkeys. We found that GnRH neurons were functionally immature, exhibiting little fluctuation in intracellular calcium ([Ca(2+)](i)) and sparse pulses of GnRH peptide release in the first 12 d in vitro (div). By 14-18 div, GnRH neurons exhibited periodic [Ca(2+)](i) oscillations, synchronizing at approximately 60-min intervals and GnRH pulses occurred at approximately 60-min intervals. Interestingly, the total GnRH peptide release further increased after 18 div. Measurement of GnRH mRNA and gene CpG methylation status at 0, 14, and 20 div indicated that mRNA levels significantly (P < 0.05) increased between 14 and 20 div, just as maximal decapeptide release was observed. By bisulfite sequencing across a 5' CpG island of the GnRH gene, we further found that methylation at eight of 14 CpG sites significantly (P < 0.05) decreased between 0 and 20 div. These data indicate that epigenetic differentiation occurs during GnRH neuronal development and suggest that increased GnRH gene expression and decreased CpG methylation status are molecular phenotypes of mature GnRH neurons. To our knowledge, this is the first report that developmental DNA demethylation occurs in postmitotic neurons toward a stable neuronal phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.