ArticleAmerican journal of physiology. Renal physiology2011
P2X7 receptors mediate deleterious renal epithelial-fibroblast cross talk.
Article in American journal of physiology. Renal physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Ameliorating Acute Kidney Injury Induced by Ischemia-Reperfusion by Targeting Purine Metabolism.International journal of molecular sciences · 2025Article
- Purinoreceptor P2X7 in Extracellular ATP-Mediated Inflammation through the Spectrum of Kidney Diseases and Kidney Transplantation.Journal of the American Society of Nephrology : JASN · 2025Review
- Ion channels as a therapeutic target for renal fibrosis.Frontiers in physiology · 2022Review
- P2X7 Receptors: An Untapped Target for the Management of Cardiovascular Disease.Arteriosclerosis, thrombosis, and vascular biology · 2021Review
- The Yin and Yang of Alarmins in Regulation of Acute Kidney Injury.Frontiers in medicine · 2020Review
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Searching Novel Therapeutic Targets for Scleroderma: P2X7-Receptor Is Up-regulated and Promotes a Fibrogenic Phenotype in Systemic Sclerosis Fibroblasts.Frontiers in pharmacology · 2017Article
- The dark side of extracellular ATP in kidney diseases.Journal of the American Society of Nephrology : JASN · 2015Review
- P2X7 receptor inhibition protects against ischemic acute kidney injury in mice.American journal of physiology. Cell physiology · 2015Article
- Exaggerated renal fibrosis in P2X4 receptor-deficient mice following unilateral ureteric obstruction.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2014Article
- Purinergic signalling in the kidney in health and disease.Purinergic signalling · 2014Review
- An in vitro assessment of fibroblast and osteoblast response to alendronate-modified titanium and the potential for decreasing fibrous encapsulation.Tissue engineering. Part A · 2013Article
- Improving outcomes of acute kidney injury using mouse renal progenitor cells alone or in combination with erythropoietin or suramin.Stem cell research & therapy · 2013Article
- Purine receptor mediated actin cytoskeleton remodeling of human fibroblasts.Cell calcium · 2013Article
- Necrotic renal epithelial cell inhibits renal interstitial fibroblast activation: role of protein tyrosine phosphatase 1B.American journal of physiology. Renal physiology · 2013Article
- The relationship between P2X4 and P2X7: a physiologically important interaction?Frontiers in physiology · 2013Article
- Emerging key roles for P2X receptors in the kidney.Frontiers in physiology · 2013Article
- Autophagy protects against necrotic renal epithelial cell-induced death of renal interstitial fibroblasts.American journal of physiology. Renal physiology · 2012Article
- P2X7 purinoceptor alterations in dystrophic mdx mouse muscles: relationship to pathology and potential target for treatment.Journal of cellular and molecular medicine · 2012Article
- ERK pathway mediates P2X7 expression and cell death in renal interstitial fibroblasts exposed to necrotic renal epithelial cells.American journal of physiology. Renal physiology · 2011Article
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6 authors at 1 institution in 1 country.
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Abstract
Peritubular fibroblasts in the kidney are the major erythropoietin-producing cells and also contribute to renal repair following acute kidney injury (AKI). Although few fibroblasts were observed in the interstitium adjacent to damaged tubular epithelium in the early phase of AKI, the underlying mechanism by which their numbers were reduced remains unknown. In this study, we tested the hypothesis that damaged renal epithelial cells directly induce renal interstitial fibroblast death by releasing intracellular ATP and activating purinergic signaling. Exposure of a cultured rat renal interstitial fibroblast cell line (NRK-49F) to necrotic renal proximal tubular cells (RPTC) lysate or supernatant induced NRK-49F cell death by apoptosis and necrosis. Depletion of ATP with apyrase or inhibition of the P2X purinergic receptor with pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid blocked the deleterious effect of necrotic RPTC supernatant. The P2X7 receptor, an ATP-sensitive purinergic receptor, was not detected in cultured NRK-49F cells but was inducible by necrotic RPTC supernatant. Treatment with A438079, a highly selective P2X7 receptor inhibitor, or knockdown of the P2X7 receptor with small interference RNA diminished renal fibroblast death induced by necrotic RPTC supernatant. Conversely, overexpression of the P2X7 receptor potentiated this response. Collectively, these findings provide strong evidence that damaged renal epithelial cells can directly induce the death of renal interstitial fibroblasts by ATP activation of the P2X7 receptor.
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