ArticleAmerican journal of physiology. Renal physiology2010
Internalization of UT-A1 urea transporter is dynamin dependent and mediated by both caveolae- and clathrin-coated pit pathways.
Article in American journal of physiology. Renal physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Actin cytoskeleton and associated myosin motors within the renal epithelium.American journal of physiology. Renal physiology · 2024Review
- E3 ligase MDM2 mediates urea transporter-A1 ubiquitination under either constitutive or stimulatory conditions.American journal of physiology. Renal physiology · 2019Article
- Identification of UT-A1- and AQP2-interacting proteins in rat inner medullary collecting duct.American journal of physiology. Cell physiology · 2018Article
- RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.Frontiers in physiology · 2015Article
- Solenopsin A and analogs exhibit ceramide-like biological activity.Vascular cell · 2015Article
- Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis.The Journal of biological chemistry · 2014Article
- Activation of the cAMP/PKA pathway induces UT-A1 urea transporter monoubiquitination and targets it for lysosomal degradation.American journal of physiology. Renal physiology · 2013Article
- Small GTPase Rab14 down-regulates UT-A1 urea transport activity through enhanced clathrin-dependent endocytosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- WNK4 inhibition of ENaC is independent of Nedd4-2-mediated ENaC ubiquitination.American journal of physiology. Renal physiology · 2013Article
- New advances in urea transporter UT-A1 membrane trafficking.International journal of molecular sciences · 2013Review
- EphB2 receptor forward signaling controls cortical growth cone collapse via Nck and Pak.Molecular and cellular neurosciences · 2013Article
- Dynamin2, clathrin, and lipid rafts mediate endocytosis of the apical Na/K/2Cl cotransporter NKCC2 in thick ascending limbs.The Journal of biological chemistry · 2012Article
- Forskolin stimulation promotes urea transporter UT-A1 ubiquitination, endocytosis, and degradation in MDCK cells.American journal of physiology. Renal physiology · 2012Article
- Glycoforms of UT-A3 urea transporter with poly-N-acetyllactosamine glycosylation have enhanced transport activity.American journal of physiology. Renal physiology · 2012Article
- Depolymerization of cortical actin inhibits UT-A1 urea transporter endocytosis but promotes forskolin-stimulated membrane trafficking.American journal of physiology. Cell physiology · 2012Article
- The emerging physiological roles of the SLC14A family of urea transporters.British journal of pharmacology · 2011Review
- Mature N-linked glycans facilitate UT-A1 urea transporter lipid raft compartmentalization.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Nanomicelles loaded with doxorubicin and curcumin for alleviating multidrug resistance in lung cancer.International journal of nanomedicineArticle
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Dynamin is a large GTPase involved in several distinct modes of cell endocytosis. In this study, we examined the possible role of dynamin in UT-A1 internalization. The direct relationship of UT-A1 and dynamin was identified by coimmunoprecipitation. UT-A1 has cytosolic NH(2) and COOH termini and a large intracellular loop. Dynamin specifically binds to the intracellular loop of UT-A1, but not the NH(2) and COOH termini. In cell surface biotinylation experiments, coexpression of dynamin and UT-A1 in HEK293 cells resulted in a decrease of UT-A1 cell surface expression. Conversely, cells expressing dynamin mutant K44A, which is deficient in GTP binding, showed an increased accumulation of UT-A1 protein on the cell surface. Cell plasma membrane lipid raft fractionation experiments revealed that blocking endocytosis with dynamin K44A causes UT-A1 protein accumulation in both the lipid raft and nonlipid raft pools, suggesting that both caveolae- and clathrin-mediated mechanisms may be involved in the internalization of UT-A1. This was further supported by 1) small interfering RNA to knock down either caveolin-1 or μ2 reduced UT-A1 internalization in HEK293 cells and 2) inhibition of either the caveolae pathway by methyl-β-cyclodextrin or the clathrin pathway by concanavalin A caused UT-A1 cell membrane accumulation. Functionally, overexpression of dynamin, caveolin, or μ2 decreased UT-A1 urea transport activity and decreased UT-A1 cell surface expression. We conclude that UT-A1 endocytosis is dynamin-dependent and mediated by both caveolae- and clathrin-coated pit pathways.
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