ArticleNucleic acids research2011
DNA is a co-factor for its own replication in Xenopus egg extracts.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Use of Xenopus Egg Extracts to Study the Effects of Topoisomerase Poisons During Vertebrate DNA Replication.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Visualizing the dynamics of DNA replication and repair at the single-molecule level.Methods in cell biology · 2024Article
- Article
- Article
- Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg Extracts.Methods in enzymology · 2017Article
- Two-Stage Synapsis of DNA Ends during Non-homologous End Joining.Molecular cell · 2016Article
- DNA replication origin activation in space and time.Nature reviews. Molecular cell biology · 2015Review
- FANCJ promotes DNA synthesis through G-quadruplex structures.The EMBO journal · 2014Article
- Bringing single-molecule spectroscopy to macromolecular protein complexes.Trends in biochemical sciences · 2013Review
- Single-molecule analysis of DNA replication in Xenopus egg extracts.Methods (San Diego, Calif.) · 2012Review
- Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Uncoupling of sister replisomes during eukaryotic DNA replication.Molecular cell · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Soluble Xenopus egg extracts efficiently replicate added plasmids using a physiological mechanism, and thus represent a powerful system to understand vertebrate DNA replication. Surprisingly, DNA replication in this system is highly sensitive to plasmid concentration, being undetectable below ∼10 pM and highly efficient above ∼75 pM. DNA replication at the high plasmid concentration does not require plasmid-plasmid contacts, since replication is not inhibited when plasmids are immobilized in agarose prior to addition of egg extract. The absence of replication at low plasmid concentration is due to a defect in the assembly of pre-replication complexes (pre-RCs). pre-RC assembly requires contact-independent communication between plasmids. Our results show that in Xenopus egg extracts, aggregation of multiple replication forks is not required for efficient replication of plasmid DNA, and they suggest that DNA functions as a co-factor for its own duplication.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.