ArticleMolecular therapy : the journal of the American Society of Gene Therapy2011
Targeted gene-and-host progenitor cell therapy for nonunion bone fracture repair.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 84 citations in OpenAlex.
- State of the Nonunion: A review of the latest literature.Orthopedic reviews · 2025Article
- The current status of various preclinical therapeutic approaches for tendon repair.Annals of medicine · 2024Review
- Gene Therapy for Regenerative Medicine.Pharmaceutics · 2023Review
- iPSC-neural crest derived cells embedded in 3D printable bio-ink promote cranial bone defect repair.Scientific reports · 2022Article
- Applications of Ultrasound-Mediated Gene Delivery in Regenerative Medicine.Bioengineering (Basel, Switzerland) · 2022Review
- Mesenchymal stem cells overexpressing BMP-9 by CRISPR-Cas9 present high in vitro osteogenic potential and enhance in vivo bone formation.Gene therapy · 2021Article
- Neural crest-derived mesenchymal progenitor cells enhance cranial allograft integration.Stem cells translational medicine · 2021Article
- Basic Research on Tendon Repair: Strategies, Evaluation, and Development.Frontiers in medicine · 2021Review
- Emerging local delivery strategies to enhance bone regeneration.Biomedical materials (Bristol, England) · 2020Review
- Review
- Biomaterial-Guided Gene Delivery for Musculoskeletal Tissue Repair.Tissue engineering. Part B, Reviews · 2017Review
- In situ bone tissue engineering via ultrasound-mediated gene delivery to endogenous progenitor cells in mini-pigs.Science translational medicine · 2017Article
- Bone morphogenetic protein 9 stimulates callus formation in osteoporotic rats during fracture healing.Molecular medicine reports · 2017Article
- Surgically‑induced mouse models in the study of bone regeneration: Current models and future directions (Review).Molecular medicine reports · 2017Review
- Human Induced Pluripotent Stem Cells Differentiate Into Functional Mesenchymal Stem Cells and Repair Bone Defects.Stem cells translational medicine · 2016Article
- Gene therapy for bone tissue engineering.Tissue engineering and regenerative medicine · 2016Article
- PTH Induces Systemically Administered Mesenchymal Stem Cells to Migrate to and Regenerate Spine Injuries.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Hydroxyapatite and demineralized calf fetal growth plate effects on bone healing in rabbit model.Journal of orthopaedics and traumatology : official journal of the Italian Society of Orthopaedics and Traumatology · 2015Article
- Ordinary and Activated Bone Grafts: Applied Classification and the Main Features.BioMed research international · 2015Review
- Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine.Annals of biomedical engineering · 2014Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Nonunion fractures present a challenge to orthopedics with no optimal solution. In-vivo DNA electroporation is a gene-delivery technique that can potentially accelerate regenerative processes. We hypothesized that in vivo electroporation of an osteogenic gene in a nonunion radius bone defect site would induce fracture repair. Nonunion fracture was created in the radii of C3H/HeN mice, into which a collagen sponge was placed. To allow for recruitment of host progenitor cells (HPCs) into the implanted sponge, the mice were housed for 10 days before electroporation. Mice were electroporated with either bone morphogenetic protein 9 (BMP-9) plasmid, Luciferase plasmid or injected with BMP-9 plasmid but not electroporated. In vivo bioluminescent imaging indicated that gene expression was localized to the defect site. Microcomputed tomography (µCT) and histological analysis of murine radii electroporated with BMP-9 demonstrated bone formation bridging the bone gap, whereas in the control groups the defect remained unbridged. Population of the implanted collagen sponge by HPCs transfected with the injected plasmid following electroporation was noted. Our data indicate that regeneration of nonunion bone defect can be attained by performing in vivo electroporation with an osteogenic gene combined with recruitment of HPCs. This gene therapy approach may pave the way for regeneration of other skeletal tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.