ArticleThe EMBO journal2010
Hen1 is required for oocyte development and piRNA stability in zebrafish.
Article in The EMBO journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 98 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
98 citing papers in PubMed, 178 citations in OpenAlex.
- Primordial Germ Cell Development in Teleosts: Their Origin, Fate and Role in Sex Determination.Stem cell reviews and reports · 2026Review
- HENMT1: an RNA methyltransferase in biology and disease.Journal of Zhejiang University. Science. B · 2026Review
- New insights into the molecular basis of gametogenesis in the hybridogenetic water frog Pelophylax esculentus.Scientific reports · 2026Article
- MicroRNA chemical modifications in post-transcriptional gene silencing and human diseases.Molecular therapy. Nucleic acids · 2025Review
- Meioc-Piwil1 complexes regulate rRNA transcription for differentiation of spermatogonial stem cells.eLife · 2025Article
- Emerging role of small RNAs in inflammatory bowel disease and associated colorectal cancer (Review).International journal of molecular medicine · 2025Review
- The piRNA protein Asz1 is essential for germ cell and gonad development in zebrafish and exhibits differential necessities in distinct types of germ granules.PLoS genetics · 2025Article
- Recent advances of PIWI-interacting RNA in cardiovascular diseases.Clinical and translational medicine · 2024Review
- Review
- Article
- Non-gonadal somatic piRNA pathways ensure sexual differentiation, larval growth, and wing development in silkworms.PLoS genetics · 2023Article
- Small RNAs: An expanding world with therapeutic promises.Fundamental research · 2023Review
- Small RNA modifications: regulatory molecules and potential applications.Journal of hematology & oncology · 2023Review
- PiRNA Obtained through Liquid Biopsy as a Possible Cancer Biomarker.Diagnostics (Basel, Switzerland) · 2023Review
- MicroRNA turnover: a tale of tailing, trimming, and targets.Trends in biochemical sciences · 2023Review
- Accurate quantification of 3'-terminal 2'-O-methylated small RNAs by utilizing oxidative deep sequencing and stem-loop RT-qPCR.Frontiers of medicine · 2022Article
- Isolation and Processing of Bovine Oocytes for Small RNA Sequencing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Terminal modification, sequence, length, and PIWI-protein identity determine piRNA stability.Molecular cell · 2021Article
- PiRNA pathway in the cardiovascular system: a novel regulator of cardiac differentiation, repair and regeneration.Journal of molecular medicine (Berlin, Germany) · 2021Review
- The epitranscriptome of small non-coding RNAs.Non-coding RNA research · 2021Review
38 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Piwi-interacting RNAs (piRNAs) are germ line-specific small RNA molecules that have a function in genome defence and germ cell development. They associate with a specific class of Argonaute proteins, named Piwi, and function through an RNA interference-like mechanism. piRNAs carry a 2'-O-methyl modification at their 3' end, which is added by the Hen1 enzyme. We show that zebrafish hen1 is specifically expressed in germ cells and is essential for maintaining a female germ line, whereas it is dispensable in the testis. Hen1 protein localizes to nuage through its C-terminal domain, but is not required for nuage formation. In hen1 mutant testes, piRNAs become uridylated and adenylated. Uridylation frequency is highest on retro-transposon-derived piRNAs and is accompanied by decreased piRNA levels and mild derepression of transposon transcripts. Altogether, our data suggest the existence of a uridylation-mediated 3'-5' exonuclease activity acting on piRNAs in zebrafish germ cells, which is counteracted by nuage-bound Hen1 protein. This system discriminates between piRNA targets and is required for ovary development and fully efficient transposon silencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.