ArticleBiomaterials2011
The use of micropatterning to control smooth muscle myosin heavy chain expression and limit the response to transforming growth factor β1 in vascular smooth muscle cells.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Smooth muscle ultrastructure - still shrouded in mystery?Protoplasma · 2026Review
- The Effects of Biomimetic Surface Topography on Vascular Cells: Implications for Vascular Conduits.Advanced healthcare materials · 2024Review
- Engineering of the microenvironment to accelerate vascular regeneration.Trends in molecular medicine · 2023Review
- Geometric Constraints Regulate Energy Metabolism and Cellular Contractility in Vascular Smooth Muscle Cells by Coordinating Mitochondrial DNA Methylation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Mechanical programming of arterial smooth muscle cells in health and ageing.Biophysical reviews · 2021Review
- A simple method to align cells on 3D hydrogels using 3D printed molds.Biomedical engineering advances · 2021Article
- Construction of vascular graft with circumferentially oriented microchannels for improving artery regeneration.Biomaterials · 2020Article
- Tethering transforming growth factor β1 to soft hydrogels guides vascular smooth muscle commitment from human mesenchymal stem cells.Acta biomaterialia · 2020Article
- Biomimetic human small muscular pulmonary arteries.Science advances · 2020Article
- Surface modification of uniaxial cyclic strain cell culture platform with temperature-responsive polymer for cell sheet detachment.Journal of materials chemistry. B · 2015Article
- Vascular smooth muscle cell functional contractility depends on extracellular mechanical properties.Journal of biomechanics · 2015Article
- Vascular smooth muscle cell culture in microfluidic devices.Biomicrofluidics · 2014Article
- Syndecan-1 regulates vascular smooth muscle cell phenotype.PloS one · 2014Article
- A Simple Nanoscale Interface Directs Alignment of a Confluent Cell Layer on Oxide and Polymer Surfaces.Journal of materials chemistry. B · 2013Article
- Perivascular cells in blood vessel regeneration.Biotechnology journal · 2013Review
- Photoreactive elastin-like proteins for use as versatile bioactive materials and surface coatings.Journal of materials chemistry · 2012Article
- Article
- Vascular smooth muscle contractility depends on cell shape.Integrative biology : quantitative biosciences from nano to macro · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In the healthy artery, contractile vascular smooth muscle cells (VSMCs) have an elongated shape and are highly aligned but transition to a synthetic phenotype in culture, while additionally becoming well spread and randomly organized. Thus, controlling VSMC phenotype is a challenge in tissue engineering. In this study, we investigated the effects of micropatterning on contractile protein expression in VSMCs at low and high passage and in the presence of transforming growth factor beta 1 (TGFβ1). Micropatterning led to significantly decreased cell area, increased elongation, and increased alignment compared to non-patterned VSMCs independent of passage number. In the presence of serum, micropatterning led to increased smooth muscle myosin heavy chain (SM-MHC) and α-actin expression in low passage VSMCs, but had no effect on high passage VSMCs. Micropatterning was as effective as TGFβ1 in up-regulating SM-MHC at low passage; however, micropatterning limited VSMC response to TGFβ1 at both low and high passage. Investigation of TGFβ receptor 1 revealed higher expression in non-patterned VSMCs compared to patterned at high passage. Our studies demonstrate that micropatterning is an important regulator of SM-MHC expression in contractile VSMCs and that it may provide a mechanism for phenotype stabilization in the presence of growth factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.