Evidence map›Paper›PMID 20858455›Full record

ArticleActa tropica2011

Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia.

Li-Li Chan, Joon-Wah Mak, Yoon-Tong Low, Thuan-Tzen Koh, Init Ithoi, Shar Mariam Mohamed

Abstract read
In one paragraph

Article in Acta tropica, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Review
  2. Article
  3. Isolation and molecular identification ofParasite epidemiology and control · 2021
    Article
  4. Article
  5. Article
  6. Article
  7. Acanthamoeba in Southeast Asia - Overview and Challenges.The Korean journal of parasitology · 2019
    Review
  8. Article
  9. The many lives of nontuberculous mycobacteria.Journal of bacteriology · 2018
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Airborne Microalgae: Insights, Opportunities, and Challenges.Applied and environmental microbiology · 2016
    Review
  15. Article
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Li-Li ChanDepartment of Postgraduate and Research, International Medical University, No. 126, Jalan 19/155B, Bukit Jalil, 57000 Kuala Lumpur, Malaysia. chanll2003@yahoo.com
Joon-Wah Mak
Yoon-Tong Low
Thuan-Tzen Koh
Init Ithoi
Shar Mariam Mohamed

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During a study on the quality of the indoor environment, Acanthamoeba spp. were detected in 20 out of 87 dust samples collected from air-conditioners installed in a four-story campus building located in Kuala Lumpur, Malaysia. Twenty-one cloned Acanthamoeba isolates designated as IMU1 to IMU21 were established from the positive primary cultures. Five species were identified from the 16 isolates according to the morphological criteria of Pussard and Pons; i.e. A. castellanii, A. culbertsoni, A. griffini, A. hatchetti and A. polyphaga. Species identities for the remaining five isolates (IMU4, IMU5, IMU15, IMU20 and IMU21), however, could not be determined morphologically. At genotypic characterization, these isolates were placed into T3 (IMU14); T5 (IMU16 and IMU17) and T4 (all the remaining isolates). To predict the potential pathogenicity of these Acanthamoeba isolates, thermo- and osmotolerance tests were employed; many isolates were predicted as potential human pathogens based on the outcome of these tests. This is the first time potentially pathogenic Acanthamoeba have been isolated from air-conditioners in Malaysia.

Indexed as

Air ConditioningDustEnvironmental MicrobiologyAcanthamoebaCell SurvivalCluster AnalysisDNA, ProtozoanDNA, RibosomalGenes, rRNAHot TemperatureHumansMalaysiaMicroscopyMolecular Sequence DataOsmotic PressurePhylogenyDNA, ProtozoanDNA, RibosomalDustRNA, ProtozoanRNA, Ribosomal, 18S

Identifiers

PMID20858455
PMCPMC7117219

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.