ArticleBiophysical journal2010
Multi-image colocalization and its statistical significance.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 57 citations in OpenAlex.
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- The Role of Hydrogen Sulfide in iNOS and APP Localization and Expression in Neurons and Glial Cells Under Traumatic Effects: An Experimental Study with Bioinformatics Analysis and Biomodeling.International journal of molecular sciences · 2024Article
- MultiMatch: geometry-informed colocalization in multi-color super-resolution microscopy.Communications biology · 2024Article
- Caveolae-associated cAMP/CaJournal of molecular and cellular cardiology · 2023Article
- The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy.International journal of molecular sciences · 2023Article
- Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis.International journal of molecular sciences · 2023Article
- NO-Dependent Mechanisms of p53 Expression and Cell Death in Rat's Dorsal Root Ganglia after Sciatic-Nerve Transection.Biomedicines · 2022Article
- Analysis of conditional colocalization relationships and hierarchies in three-color microscopy images.The Journal of cell biology · 2022Article
- Modifying the mMolecular psychiatry · 2021Article
- Functional and evolutionary analysis of the Arabidopsis 4R-MYB protein SNAPc4 as part of the SNAP complex.Plant physiology · 2021Article
- MACC1 regulates clathrin-mediated endocytosis and receptor recycling of transferrin receptor and EGFR in colorectal cancer.Cellular and molecular life sciences : CMLS · 2021Article
- Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation.iScience · 2021Article
- EzColocalization: An ImageJ plugin for visualizing and measuring colocalization in cells and organisms.Scientific reports · 2018Article
- Complexes of α6β4 integrin and vimentin act as signaling hubs to regulate epithelial cell migration.Journal of cell science · 2018Article
- Article
- Quantitative Measurement of Naïve T Cell Association With Dendritic Cells, FRCs, and Blood Vessels in Lymph Nodes.Frontiers in immunology · 2018Article
- Modeling, validation and verification of three-dimensional cell-scaffold contacts from terabyte-sized images.BMC bioinformatics · 2017Article
- A Method for Quantifying Molecular Interactions Using Stochastic Modelling and Super-Resolution Microscopy.Scientific reports · 2017Article
- MatCol: a tool to measure fluorescence signal colocalisation in biological systems.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Accurately localizing molecules within the cell is one of main tasks of modern biology, and colocalization analysis is one of its principal and most often used tools. Despite this popularity, interpretation is often uncertain because colocalization between two or more images is rarely analyzed to determine whether the observed values could have occurred by chance. To address this, we have developed a robust methodology, based on Monte Carlo randomization, to measure the statistical significance of a colocalization. The method works with voxel-based, intensity-based, object-based, and nearest-neighbor metrics. We extend all of these to measure colocalization in images with three colors. We also introduce three new metrics; blob colocalization, where the blob consists of a local maximum surrounded by a three-dimensional group of voxels; cluster diameter, to measure the clustering of fluorophores in three or more images; and the intercluster distance to measure the distance between these clusters. The robustness of these metrics was tested by varying the image thresholds over a broad range, which produced no change in the statistical significance of the colocalizations. A comparison of blob colocalization with voxel and Manders colocalization metrics shows that the different measures produce consistent results with similar values for significance and nonsignificance. Using our methodology, we are able to determine not only whether the labeled molecules colocalize with a probability greater than chance, but also whether they are sequestrated into different compartments. The program, written in C++, is freely available as source, as well as in a Linux version.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.