ArticleBiophysical journal2010
Salt concentration effects on equilibrium melting curves from DNA microarrays.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Strategies in the optimization of DNA hybridization conditions and its role in electrochemical detection of dengue virus (DENV) using response surface methodology (RSM).RSC advances · 2023Article
- Molecular contacts in the Cren7-DNA complex: A quantitative investigation for electrostatic interaction.Biophysical journal · 2023Article
- Functionalizing Fibrin Hydrogels with Thermally Responsive Oligonucleotide Tethers for On-Demand Delivery.Bioengineering (Basel, Switzerland) · 2022Article
- Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration.Biosensors · 2021Article
- Thermodynamic framework to assess low abundance DNA mutation detection by hybridization.PloS one · 2017Article
- Article
- SPR and SPR Imaging: Recent Trends in Developing Nanodevices for Detection and Real-Time Monitoring of Biomolecular Events.Sensors (Basel, Switzerland) · 2016Review
- Surface vs. solution hybridization: effects of salt, temperature, and probe type.Chemical communications (Cambridge, England) · 2015Article
- Crowding-induced cooperativity in DNA surface hybridization.Scientific reports · 2015Article
- Sources of high variance between probe signals in Affymetrix short oligonucleotide microarrays.Sensors (Basel, Switzerland) · 2013Article
- Targeted resequencing of HIV variants by microarray thermodynamics.Nucleic acids research · 2013Article
- Physico-chemical foundations underpinning microarray and next-generation sequencing experiments.Nucleic acids research · 2013Review
- Melting thermodynamics of reversible DNA/ligand complexes at interfaces.Journal of the American Chemical Society · 2012Article
- Probing hybridization parameters from microarray experiments: nearest-neighbor model and beyond.Nucleic acids research · 2012Article
- Thermostable DNA immobilization and temperature effects on surface hybridization.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Nucleic acid-based diagnostics for infectious diseases in public health affairs.Frontiers of medicine · 2012Review
- Accurate prediction of binding thermodynamics for DNA on surfaces.The journal of physical chemistry. B · 2011Article
- Kinetic mechanisms in morpholino-DNA surface hybridization.Journal of the American Chemical Society · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA microarrays find applications in an increasing number of domains where more quantitative results are required. DNA being a charged polymer, the repulsive interactions between the surface of the microarray and the targets in solution are increasing upon hybridization. Such electrostatic penalty is generally reduced by increasing the salt concentration. In this article, we present equilibrium-melting curves obtained from dedicated physicochemical experiments on DNA microarrays in order to get a better understanding of the electrostatic penalty incurred during the hybridization reaction at the surface. Various salt concentrations have been considered and deviations from the commonly used Langmuir adsorption model are experimentally quantified for the first time in agreement with theoretical predictions.
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