ArticleBiophysical journal2010
Second harmonic generation microscopy probes different states of motor protein interaction in myofibrils.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Probing relaxed myosin states in hypertrophic cardiomyopathy by second harmonic-generation microscopy.eLife · 2026Article
- Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generation.Life science alliance · 2023Article
- Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Changes in the crystallographic structures of cardiac myosin filaments detected by polarization-dependent second harmonic generation microscopy.Biomedical optics express · 2019Article
- Polarization-resolved second harmonic microscopy of skeletal muscle in sepsis.Biomedical optics express · 2018Article
- Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach.Light, science & applications · 2018Article
- μMAPPS: a novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure.Scientific reports · 2017Article
- Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution.Biomedical optics express · 2016Article
- Multimodal SHG-2PF Imaging of Microdomain Ca2+-Contraction Coupling in Live Cardiac Myocytes.Circulation research · 2016Review
- Visualization of mouse neuronal ganglia infected by Herpes Simplex Virus 1 (HSV-1) using multimodal non-linear optical microscopy.PloS one · 2014Article
- Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine.Journal of the Royal Society, Interface · 2013Review
- Imaging the bipolarity of myosin filaments with Interferometric Second Harmonic Generation microscopy.Biomedical optics express · 2013Article
- Measurement of orientation and susceptibility ratios using a polarization-resolved second-harmonic generation holographic microscope.Biomedical optics express · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The second harmonic generation (SHG) signal intensity sourced from skeletal muscle myosin II strongly depends on the polarization of the incident laser beam relative to the muscle fiber axis. This dependence is related to the second-order susceptibility χ((2)), which can be described by a single component ratio γ under generally assumed symmetries. We precisely extracted γ from SHG polarization dependence curves with an extended focal field model. In murine myofibrillar preparations, we have found two distinct polarization dependencies: With the actomyosin system in the rigor state, γ(rig) has a mean value of γ(rig) = 0.52 (SD = 0.04, n = 55); in a relaxed state where myosin is not bound to actin, γ(rel) has a mean value of γ(rel) = 0.24 (SD = 0.07, n = 70). We observed a similar value in an activated state where the myosin power stroke was pharmacologically inhibited using N-benzyl-p-toluene sulfonamide. In summary, different actomyosin states can be visualized noninvasively with SHG microscopy. Specifically, SHG even allows us to distinguish different actin-bound states of myosin II using γ as a parameter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.