ArticleBiophysical journal2010
Fluorescence anisotropy reveals order and disorder of protein domains in the nuclear pore complex.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 63 citations in OpenAlex.
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- Observing biological spatio-angular structures and dynamics with statistical image reconstruction and polarized fluorescence microscopy.bioRxiv : the preprint server for biology · 2025Article
- Dsg2 ectodomain organization increases throughout desmosome assembly.Cell adhesion & migration · 2024Article
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.PLoS computational biology · 2024Article
- A survey of the specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport.Nucleus (Austin, Tex.) · 2023Article
- Extracellular matrix sensing via modulation of orientational order of integrins and F-actin in focal adhesions.Life science alliance · 2023Article
- Improving the hole picture: towards a consensus on the mechanism of nuclear transport.Biochemical Society transactions · 2023Article
- Dynamic molecular mechanism of the nuclear pore complex permeability barrier.bioRxiv : the preprint server for biology · 2023Article
- Article
- Unraveling the geometry of complex protein organizations by polarized fluorescence imaging.Biophysical journal · 2022Article
- Fluorescence polarization modulation super-resolution imaging provides refined dynamics orientation processes in biological samples.Light, science & applications · 2022Article
- Pressure and temperature dependence of fluorescence anisotropy of green fluorescent protein.RSC advances · 2022Article
- Article
- Spatio-angular fluorescence microscopy III. Constrained angular diffusion, polarized excitation, and high-NA imaging.Journal of the Optical Society of America. A, Optics, image science, and vision · 2020Article
- High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.Methods and applications in fluorescence · 2020Review
- Kinetics of Histidine-Tagged Protein Association to Nickel-Decorated Liposome Surfaces.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Spatio-angular fluorescence microscopy I. Basic theory.Journal of the Optical Society of America. A, Optics, image science, and vision · 2019Article
- Age-dependent deterioration of nuclear pore assembly in mitotic cells decreases transport dynamics.eLife · 2019Article
- Homotransfer of FRET Reporters for Live Cell Imaging.Biosensors · 2018Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We present a new approach for studying individual protein domains within the nuclear pore complex (NPC) using fluorescence polarization microscopy. The NPC is a large macromolecular complex, the size and complexity of which presents experimental challenges. Using fluorescence anisotropy and exploiting the symmetry of the NPC and its organization in the nuclear envelope, we have resolved order and disorder of individual protein domains. Fluorescently tagging specific domains of individual nucleoporins revealed both rigid and flexible domains: the tips of the FG domains are disordered, whereas the NPC-anchored domains are ordered. Our technique allows the collection of structural information in vivo, providing the ability to probe the organization of protein domains within the NPC. This has particular relevance for the FG domain nucleoporins, which are crucial for nucleocytoplasmic transport.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.