ArticleBMC neuroscience2010
Isolation of detergent resistant microdomains from cultured neurons: detergent dependent alterations in protein composition.
Article in BMC neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
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- Neuronal Signaling by Thy-1 in Nanodomains With Specific Ganglioside Composition: Shall We Open the Door to a New Complexity?Frontiers in cell and developmental biology · 2019Article
- Sphingolipids and lipid rafts: Novel concepts and methods of analysis.Chemistry and physics of lipids · 2018Review
- Hippocampal encoding of interoceptive context during fear conditioning.Translational psychiatry · 2017Article
- The activities of LDL Receptor-related Protein-1 (LRP1) compartmentalize into distinct plasma membrane microdomains.Molecular and cellular neurosciences · 2016Article
- Budding Yeast: An Ideal Backdrop forFrontiers in cell and developmental biology · 2016Review
- Human cell adhesion molecules: annotated functional subtypes and overrepresentation of addiction-associated genes.Annals of the New York Academy of Sciences · 2015Review
- Exosomal sorting of the viral oncoprotein LMP1 is restrained by TRAF2 association at signalling endosomes.Journal of extracellular vesicles · 2015Article
- Raft-like membranes from the trans-Golgi network and endosomal compartments.Nature protocols · 2013Article
- Competition between homodimerization and cholesterol binding to the C99 domain of the amyloid precursor protein.Biochemistry · 2013Article
- 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins.Cell · 2013Article
- Proteome analysis of Cry4Ba toxin-interacting Aedes aegypti lipid rafts using geLC-MS/MS.Journal of proteome research · 2012Article
- Identification of novel γ-secretase-associated proteins in detergent-resistant membranes from brain.The Journal of biological chemistry · 2012Article
- Surface expression and limited proteolysis of ADAM10 are increased by a dominant negative inhibitor of dynamin.BMC cell biology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMembrane rafts are small highly dynamic sterol- and sphingolipid-enriched membrane domains that have received considerable attention due to their role in diverse cellular functions. More recently the involvement of membrane rafts in neuronal processes has been highlighted since these specialized membrane domains have been shown to be involved in synapse formation, neuronal polarity and neurodegeneration. Detergent resistance followed by gradient centrifugation is often used as first step in screening putative membrane raft components. Traditional methods of raft isolation employed the nonionic detergent Triton X100. However successful separation of raft from non-raft domains in cells is dependent on matching the detergent used for raft isolation to the specific tissue under investigation.
resultsWe report here the isolation of membrane rafts from primary neuronal culture using a panel of different detergents that gave rise to membrane fractions that differed in respect to cholesterol and protein content. In addition, proteomic profiling of neuronal membrane rafts isolated with different detergents, Triton X100 and CHAPSO, revealed heterogeneity in their protein content.
conclusionsThese data demonstrate that appropriate selection of detergent for raft isolation is an important consideration for investigating raft protein composition of cultured neurons.
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