ArticleBiochemistry2010
SufD and SufC ATPase activity are required for iron acquisition during in vivo Fe-S cluster formation on SufB.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- The structure of the SufBCbioRxiv : the preprint server for biology · 2026Article
- Coordination of the Fe-S cluster biogenesis network by the sRNA RyhB inRNA biology · 2025Article
- A minimal SufBMicrobiology spectrum · 2025Article
- Akkermansia muciniphila MucNature communications · 2025Article
- Volumetric Engineered 3D Drug Reservoir Against Diabetic Implant Infection via Cuproptosis-Like Bacterial Death and Hunger-Triggered Maintenance of Mitochondrial Integrity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- SmsB-SmsC machinery inApplied and environmental microbiology · 2025Article
- Characterization of a mechanistic connection between persulfide transfer and ATP hydrolysis in the SufBC2D scaffold of the Suf Fe-S cluster assembly pathway.Metallomics : integrated biometal science · 2025Article
- Genomic insights into local-scale evolution of ocularMicrobial genomics · 2024Article
- Genome mining ofFrontiers in microbiology · 2024Article
- Research progress on the biosynthesis and delivery of iron-sulfur clusters in the plastid.Plant cell reports · 2023Review
- TheeLife · 2023Article
- Structural and Biochemical Characterization ofACS omega · 2022Article
- The role of nucleoside triphosphate hydrolase metallochaperones in making metalloenzymes.Metallomics : integrated biometal science · 2022Review
- Structural diversity of cysteine desulfurases involved in iron-sulfur cluster biosynthesis.Biophysics and physicobiology · 2022Article
- Review
- Pathways of Iron and Sulfur Acquisition, Cofactor Assembly, Destination, and Storage in Diverse Archaeal Methanogens and Alkanotrophs.Journal of bacteriology · 2021Article
- The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis.The EMBO journal · 2021Article
- Occurrence, Evolution and Specificities of Iron-Sulfur Proteins and Maturation Factors in Chloroplasts from Algae.International journal of molecular sciences · 2021Review
- Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress.BMC microbiology · 2020Article
- Fe-S cluster biogenesis by the bacterial Suf pathway.Biochimica et biophysica acta. Molecular cell research · 2020Review
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Authors and funding
5 authors.
Funding
Abstract
In vivo biogenesis of Fe-S cluster cofactors requires complex biosynthetic machinery to limit release of iron and sulfide, to protect the Fe-S cluster from oxidation, and to target the Fe-S cluster to the correct apoenzyme. The SufABCDSE pathway for Fe-S cluster assembly in Escherichia coli accomplishes these tasks under iron starvation and oxidative stress conditions that disrupt Fe-S cluster metabolism. Although SufB, SufC, and SufD are all required for in vivo Suf function, their exact roles are unclear. Here we show that SufB, SufC, and SufD, coexpressed with the SufS-SufE sulfur transfer pair, purify as two distinct complexes (SufBC(2)D and SufB(2)C(2)) that contain Fe-S clusters and FADH(2). These studies also show that SufC and SufD are required for in vivo Fe-S cluster formation on SufB. Furthermore, while SufD is dispensable for in vivo sulfur transfer, it is absolutely required for in vivo iron acquisition. Finally, we demonstrate for the first time that the ATPase activity of SufC is necessary for in vivo iron acquisition during Fe-S cluster assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.