ArticleMedical molecular morphology2010
Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry.
Article in Medical molecular morphology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- A novel murine model of autoimmune dysautonomia by α3 nicotinic acetylcholine receptor immunization.Frontiers in neuroscience · 2022Article
- Investigation of Inter- and Intratumoral Heterogeneity of Glioblastoma Using TOF-SIMS.Molecular & cellular proteomics : MCP · 2020Article
- Unsupervised machine learning using an imaging mass spectrometry dataset automatically reassembles grey and white matter.Scientific reports · 2019Article
- Lipid specific molecular ion emission as a function of the primary ion characteristics in TOF-SIMS.Journal of vacuum science and technology. B, Nanotechnology & microelectronics : materials, processing, measurement, & phenomena : JVST B · 2016Article
- Spatiotemporal lipid profiling during early embryo development of Xenopus laevis using dynamic ToF-SIMS imaging.Journal of lipid research · 2014Article
- Mass spectrometry imaging, an emerging technology in neuropsychopharmacology.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry.Nature protocols · 2013Article
- Article
- Single-cell metabolomics: changes in the metabolome of freshly isolated and cultured neurons.ACS chemical neuroscience · 2012Article
- Visualization of acetylcholine distribution in central nervous system tissue sections by tandem imaging mass spectrometry.Analytical and bioanalytical chemistry · 2012Article
- Lipid imaging with time-of-flight secondary ion mass spectrometry (ToF-SIMS).Biochimica et biophysica acta · 2011Review
- Highlighting anatomical sub-structures in rat brain tissue using lipid imaging.Analytical methods : advancing methods and applications · 2011Article
- Matrix-assisted laser desorption/ionization imaging mass spectrometry.International journal of molecular sciences · 2010Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurons have a large surface because of their long and thin neurites. This surface is composed of a lipid bilayer. Lipids have not been actively investigated so far because of some technical difficulties, although evidence from cell biology is emerging that lipids contain valuable information about their roles in the central nervous system. Recent progress in techniques, e.g., mass spectrometry, opens a new epoch of lipid research. We show herein the characteristic localization of phospholipid components in neurites by means of time-of-flight secondary ion mass spectrometry. We used explant cultures of mouse superior cervical ganglia, which are widely used by neurite investigation research. In a positive-ion detection mode, phospholipid head group molecules were predominantly detected. The ions of m/z 206.1 [phosphocholine, a common component of phosphatidylcholine (PC) and sphingomyelin (SM)] were evenly distributed throughout the neurites, whereas the ions of m/z 224.1, 246.1 (glycerophosphocholine, a part of PC, but not SM) showed relatively strong intensity on neurites adjacent to soma. In a negative-ion detection mode, fatty acids such as oleic and palmitic acids were mainly detected, showing high intensity on neurites adjacent to soma. Our results suggest that lipid components on the neuritic surface show characteristic distributions depending on neurite region.
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