ArticleMolecular biology reports2011
Isolation and characterization of a novel BcMF14 gene from Brassica campestris ssp. chinensis.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Genome-Wide Characterization ofInternational journal of molecular sciences · 2025Article
- Identification of an anther-specific promoter from a male sterile AB line in Chinese cabbage (3 Biotech · 2022Article
- Assessment of Platycodon grandiflorum germplasm resources from northern Anhui province based on ISSR analysis.Molecular biology reports · 2014Article
- Characterization of Betula platyphylla gene transcripts associated with early development of male inflorescence.Molecular biology reports · 2012Article
- Molecular cloning and its expression of trachealess gene (As-trh) during development in brine shrimp, Artemia sinica.Molecular biology reports · 2012Article
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Authors and funding
3 authors.
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Abstract
A putative RALF (rapid alkalinization factor)-like gene (GenBank accession number EF523517), named BcMF14, was isolated from Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis) by rapid amplification of cDNA ends (RACE) based on a cDNA-AFLP differential fragment exclusively expressed in fertile line. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) discovered that BcMF14 was prominently expressed in stage four and five flower buds of fertile line, no expression in vegetative structures or in sterility line. Detailed RT-PCR illuminated its strong expression in stamens. Successful suppression of BcMF14 gene expression greatly reduced the normal pollen grains. The frequency of abnormal pollen grains was 48.95% in the mutant with many shriveled pollen grains with irregular shape and some larger ones with deep hollows along the germination ditch. Pollen germination was stopped because of the severely twisted pollen tubes. These results demonstrate a potential role of the BcMF14 gene in the development of male gametogenesis in Chinese cabbage.
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