ArticlePloS one2010
Microparticles carrying Sonic hedgehog favor neovascularization through the activation of nitric oxide pathway in mice.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
47 citing papers in PubMed, 97 citations in OpenAlex.
- Intramuscular Adipose Tissue Accumulation is a Key Determinant of Limb Function in Peripheral Artery Disease.Circulation · 2026Article
- Intramuscular Adipose Tissue Accumulation is a Key Determinant of Limb Function in Peripheral Artery Disease.bioRxiv : the preprint server for biology · 2026Article
- Morphogens and Cell-Derived Structures (Exosomes and Cytonemes) as Components of the Communication Between Cells.International journal of molecular sciences · 2025Review
- Therapeutic implication of Sonic Hedgehog as a potential modulator in ischemic injury.Pharmacological reports : PR · 2023Review
- Blood Cell-Derived Microvesicles in Hematological Diseases and beyond.Biomolecules · 2022Review
- Review
- Role of Extracellular Vesicles as Potential Diagnostic and/or Therapeutic Biomarkers in Chronic Cardiovascular Diseases.Frontiers in cell and developmental biology · 2022Review
- Scaffolds and Extracellular Vesicles as a Promising Approach for Cardiac Regeneration after Myocardial Infarction.Pharmaceutics · 2020Review
- Extracellular Vesicles miRNA Cargo for Microglia Polarization in Traumatic Brain Injury.Biomolecules · 2020Review
- Shedding Light on the Role of Extracellular Vesicles in HIV Infection and Wound Healing.Viruses · 2020Review
- Immunoregulatory Roles of Extracellular Vesicles and Associated Therapeutic Applications in Lung Cancer.Frontiers in immunology · 2020Review
- Cardioprotective effect of sonic hedgehog ligand in pig models of ischemia reperfusion.Theranostics · 2020Article
- Extracellular vesicles from T cells overexpress miR-146b-5p in HIV-1 infection and repress endothelial activation.Scientific reports · 2019Article
- Role of Hedgehog Signaling in Vasculature Development, Differentiation, and Maintenance.International journal of molecular sciences · 2019Review
- Molecular Mechanisms Underpinning Microparticle-Mediated Cellular Injury in Cardiovascular Complications Associated with Diabetes.Oxidative medicine and cellular longevity · 2019Review
- Exosomes in the Regulation of Vascular Endothelial Cell Regeneration.Frontiers in cell and developmental biology · 2019Review
- Microparticles Carrying Sonic Hedgehog Are Increased in Humans with Peripheral Artery Disease.International journal of molecular sciences · 2018Article
- Sonic Hedgehog Signaling Rises to the Surface: Emerging Roles in Neocortical Development.Brain plasticity (Amsterdam, Netherlands) · 2018Review
- Extracellular Vesicles and the Application of System Biology and Computational Modeling in Cardiac Repair.Circulation research · 2018Review
- Extracellular Vesicle-Mediated Immune Regulation of Tissue Remodeling and Angiogenesis After Myocardial Infarction.Frontiers in immunology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroparticles (MPs) are vesicles released from plasma membrane upon cell activation and during apoptosis. Human T lymphocytes undergoing activation and apoptosis generate MPs bearing morphogen Shh (MPs(Shh+)) that are able to regulate in vitro angiogenesis. METHODOLOGY/PRINCIPAL
findingsHere, we investigated the ability of MPs(Shh+) to modulate neovascularization in a model of mouse hind limb ischemia. Mice were treated in vivo for 21 days with vehicle, MPs(Shh+), MPs(Shh+) plus cyclopamine or cyclopamine alone, an inhibitor of Shh signalling. Laser doppler analysis revealed that the recovery of the blood flow was 1.4 fold higher in MPs(Shh+)-treated mice than in controls, and this was associated with an activation of Shh pathway in muscles and an increase in NO production in both aorta and muscles. MPs(Shh+)-mediated effects on flow recovery and NO production were completely prevented when Shh signalling was inhibited by cyclopamine. In aorta, MPs(Shh+) increased activation of eNOS/Akt pathway, and VEGF expression, being inhibited by cyclopamine. By contrast, in muscles, MPs(Shh+) enhanced eNOS expression and phosphorylation and decreased caveolin-1 expression, but cyclopamine prevented only the effects of MPs(Shh+) on eNOS pathway. Quantitative RT-PCR revealed that MPs(Shh+) treatment increased FGF5, FGF2, VEGF A and C mRNA levels and decreased those of α5-integrin, FLT-4, HGF, IGF-1, KDR, MCP-1, MT1-MMP, MMP-2, TGFβ1, TGFβ2, TSP-1 and VCAM-1, in ischemic muscles. CONCLUSIONS/SIGNIFICANCE: These findings suggest that MPs(Shh+) may contribute to reparative neovascularization after ischemic injury by regulating NO pathway and genes involved in angiogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.