ArticlePLoS neglected tropical diseases2010
Application and validation of PFGE for serovar identification of Leptospira clinical isolates.
Article in PLoS neglected tropical diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 74 citations in OpenAlex.
- Shaping the future ofJournal of medical microbiology · 2025Review
- Diagnosis and Clinical Description of Leptospirosis Cases Identified Through Hospital-Based Active Surveillance in Puerto Rico, 2019-2021.Journal of tropical medicine · 2025Article
- Navigating cross-reactivity and host species effects in a serological assay: A case study of the microscopic agglutination test for Leptospira serology.PLoS neglected tropical diseases · 2024Article
- Isolation of VirulentMicroorganisms · 2024Article
- Application of pulsed-field gel electrophoresis for molecular identification of pathogenicIranian journal of microbiology · 2024Article
- Genus-wide Leptospira core genome multilocus sequence typing for strain taxonomy and global surveillance.PLoS neglected tropical diseases · 2019Article
- Evaluation of BOX-PCR and ERIC-PCR as Molecular Typing Tools for PathogenicDisease markers · 2018Article
- Genomic Comparison Among Global Isolates ofFrontiers in cellular and infection microbiology · 2018Article
- Characterization of Leptospira isolates from humans and the environment in Uruguay.Revista do Instituto de Medicina Tropical de Sao Paulo · 2017Article
- High-Resolution Melting Curve Analysis of the 16S Ribosomal Gene to Detect and Identify Pathogenic and SaprophyticThe American journal of tropical medicine and hygiene · 2017Article
- Genetic diversity of Leptospira in northwestern Colombia: first report of Leptospira santarosai as a recognised leptospirosis agent.Memorias do Instituto Oswaldo Cruz · 2016Article
- Profiling of Leptospira interrogans, L. santarosai, L. meyeri and L. borgpetersenii by SE-AFLP, PFGE and susceptibility testing--a continuous attempt at species and serovar differentiation.Emerging microbes & infections · 2016Article
- Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2015Article
- Genetic stability of vaccine strains by multilocus sequence typing and pulsed-field gel electrophoresis analysis: Implications for quality control of the leptospiral vaccine.Human vaccines & immunotherapeutics · 2015Article
- Leptospirosis in humans.Current topics in microbiology and immunology · 2015Review
- Isolation of Leptospira from a phocid: acute renal failure and mortality from Leptospirosis in rehabilitated northern elephant seals (Mirounga angustirostris), California, USA.Journal of wildlife diseases · 2014Article
- Epizootiological survey of small mammals as Leptospira spp. reservoirs in Eastern Croatia.Acta tropica · 2014Article
- High-resolution typing of Leptospira interrogans strains by multispacer sequence typing.Journal of clinical microbiology · 2014Article
- Interlaboratory agreement of pulsed-field gel electrophoresis identification of Leptospira serovars.The American journal of tropical medicine and hygiene · 2013Article
- Isolation and molecular characterization of Leptospira interrogans and Leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia.The American journal of tropical medicine and hygiene · 2013Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serovar identification of clinical isolates of Leptospira is generally not performed on a routine basis, yet the identity of an infecting serovar is valuable from both epidemiologic and public health standpoints. Only a small number of reference laboratories worldwide have the capability to perform the cross agglutinin absorption test (CAAT), the reference method for serovar identification. Pulsed-field gel electrophoresis (PFGE) is an alternative method to CAAT that facilitates rapid identification of leptospires to the serovar level. We employed PFGE to evaluate 175 isolates obtained from humans and animals submitted to the Centers for Disease Control and Prevention (CDC) between 1993 and 2007. PFGE patterns for each isolate were generated using the NotI restriction enzyme and compared to a reference database consisting of more than 200 reference strains. Of the 175 clinical isolates evaluated, 136 (78%) were identified to the serovar level by the database, and an additional 27 isolates (15%) have been identified as probable new serovars. The remaining isolates yet to be identified are either not represented in the database or require further study to determine whether or not they also represent new serovars. PFGE proved to be a useful tool for serovar identification of clinical isolates of known serovars from different geographic regions and a variety of different hosts and for recognizing potential new serovars.
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