ArticleMolecular & cellular proteomics : MCP2011
Laserspray ionization, a new method for protein analysis directly from tissue at atmospheric pressure with ultrahigh mass resolution and electron transfer dissociation.
Article in Molecular & cellular proteomics : MCP, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- New Processes for Ionizing Nonvolatile Compounds in Mass Spectrometry: The Road of Discovery to Current State-of-the-Art.Journal of the American Society for Mass Spectrometry · 2024Review
- Direct sub-atmospheric pressure ionization mass spectrometry: Evaporation/sublimation-driven ionization is amazing, fundamentally, and practically.Journal of mass spectrometry : JMS · 2024Review
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Mass Spectrometry Analysis of Intact Proteins from Crude Samples.Analytical chemistry · 2020Article
- Coupling matrix-assisted ionization with high resolution mass spectrometry and electron transfer dissociation to characterize intact proteins and post-translational modifications.Analytical and bioanalytical chemistry · 2018Article
- Matrix-assisted ionization vacuum for protein detection, fragmentation and PTM analysis on a high resolution linear ion trap-orbitrap platform.Analytica chimica acta · 2016Article
- "Magic" Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- MALDI FTICR IMS of Intact Proteins: Using Mass Accuracy to Link Protein Images with Proteomics Data.Journal of the American Society for Mass Spectrometry · 2015Article
- In Situ characterization of proteins using laserspray ionization on a high-performance MALDI-LTQ-Orbitrap mass spectrometer.Journal of the American Society for Mass Spectrometry · 2014Article
- Transmission geometry laserspray ionization vacuum using an atmospheric pressure inlet.Analytical chemistry · 2014Article
- Matrix assisted ionization vacuum (MAIV), a new ionization method for biological materials analysis using mass spectrometry.Molecular & cellular proteomics : MCP · 2013Article
- Laserspray and matrix-assisted ionization inlet coupled to high-field FT-ICR mass spectrometry for peptide and protein analysis.Journal of the American Society for Mass Spectrometry · 2013Article
- Laserspray ionization imaging of multiply charged ions using a commercial vacuum MALDI ion source.Analytical chemistry · 2012Article
- Matrix assisted ionization: new aromatic and nonaromatic matrix compounds producing multiply charged lipid, peptide, and protein ions in the positive and negative mode observed directly from surfaces.Journal of the American Society for Mass Spectrometry · 2012Article
- A mechanism for ionization of nonvolatile compounds in mass spectrometry: considerations from MALDI and inlet ionization.Journal of the American Society for Mass Spectrometry · 2012Article
- Probing neuropeptide signaling at the organ and cellular domains via imaging mass spectrometry.Journal of proteomics · 2012Review
- Mass Spectrometry-based Proteomics and Peptidomics for Systems Biology and Biomarker Discovery.Frontiers in biology · 2012Article
- Localization and imaging of gangliosides in mouse brain tissue sections by laserspray ionization inlet.Journal of lipid research · 2012Article
- A new approach to high sensitivity liquid chromatography-mass spectrometry of peptides using nanoflow solvent assisted inlet ionization.Journal of the American Society for Mass Spectrometry · 2012Article
- Matrix-assisted laser desorption/ionisation mass spectrometry imaging and its development for plant protein imaging.Plant methods · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Laserspray ionization (LSI) mass spectrometry (MS) allows, for the first time, the analysis of proteins directly from tissue using high performance atmospheric pressure ionization mass spectrometers. Several abundant and numerous lower abundant protein ions with molecular masses up to ∼20,000 Da were detected as highly charged ions from delipified mouse brain tissue mounted on a common microscope slide and coated with 2,5-dihydroxyacetophenone as matrix. The ability of LSI to produce multiply charged ions by laser ablation at atmospheric pressure allowed protein analysis at 100,000 mass resolution on an Orbitrap Exactive Fourier transform mass spectrometer. A single acquisition was sufficient to identify the myelin basic protein N-terminal fragment directly from tissue using electron transfer dissociation on a linear trap quadrupole (LTQ) Velos. The high mass resolution and mass accuracy, also obtained with a single acquisition, are useful in determining protein molecular weights and from the electron transfer dissociation data in confirming database-generated sequences. Furthermore, microscopy images of the ablated areas show matrix ablation of ∼15 μm-diameter spots in this study. The results suggest that LSI-MS at atmospheric pressure potentially combines speed of analysis and imaging capability common to matrix-assisted laser desorption/ionization and soft ionization, multiple charging, improved fragmentation, and cross-section analysis common to electrospray ionization.
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