ArticleRNA (New York, N.Y.)2010
TbRGG2 facilitates kinetoplastid RNA editing initiation and progression past intrinsic pause sites.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- How Binding Site Flexibility Promotes RNA Scanning by TbRGG2 RRM: A Molecular Dynamics Simulation Study.Journal of chemical information and modeling · 2025Article
- c-di-GMP regulates activity of the PlzA RNA chaperone from the Lyme disease spirochete.Molecular microbiology · 2023Article
- Structural basis for guide RNA selection by the RESC1-RESC2 complex.Nucleic acids research · 2023Article
- Structure of the T. brucei kinetoplastid RNA editing substrate-binding complex core component, RESC5.PloS one · 2023Article
- Conserved and transcript-specific functions of the RESC factors, RESC13 and RESC14, in kinetoplastid RNA editing.RNA (New York, N.Y.) · 2022Article
- Complete minicircle genome of Leptomonas pyrrhocoris reveals sources of its non-canonical mitochondrial RNA editing events.Nucleic acids research · 2021Article
- Parallel monitoring of RNA abundance, localization and compactness with correlative single molecule FISH on LR White embedded samples.Nucleic acids research · 2021Article
- Site-specific and substrate-specific control of accurate mRNA editing by a helicase complex in trypanosomes.RNA (New York, N.Y.) · 2020Article
- Poly(A) binding KPAF4/5 complex stabilizes kinetoplast mRNAs in Trypanosoma brucei.Nucleic acids research · 2020Article
- Sulfonated inhibitors of the RNA editing ligases validate the essential role of the MRP1/2 proteins in kinetoplastid RNA editing.RNA (New York, N.Y.) · 2020Article
- Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.Trends in parasitology · 2020Review
- MRB10130 is a RESC assembly factor that promotes kinetoplastid RNA editing initiation and progression.RNA (New York, N.Y.) · 2019Article
- Separating the Wheat from the Chaff: RNA Editing and Selection of Translatable mRNA in Trypanosome Mitochondria.Pathogens (Basel, Switzerland) · 2019Review
- Intrinsic and regulated properties of minimally edited trypanosome mRNAs.Nucleic acids research · 2019Article
- The RNA binding activity of the first identified trypanosome protein with Z-DNA-binding domains.Scientific reports · 2019Article
- The RRM of the kRNA-editing protein TbRGG2 uses multiple surfaces to bind and remodel RNA.Nucleic acids research · 2019Article
- Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes.RNA (New York, N.Y.) · 2018Article
- Dynamic RNA holo-editosomes with subcomplex variants: Insights into the control of trypanosome editing.Wiley interdisciplinary reviews. RNA · 2018Review
- High throughput sequencing revolution reveals conserved fundamentals of U-indel editing.Wiley interdisciplinary reviews. RNA · 2018Review
- MRB7260 is essential for productive protein-RNA interactions within the RNA editing substrate binding complex during trypanosome RNA editing.RNA (New York, N.Y.) · 2018Article
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Authors and funding
5 authors.
Funding
Abstract
TbRGG2 is an essential kinetoplastid RNA editing accessory factor that acts specifically on pan-edited RNAs. To understand the mechanism of TbRGG2 action, we undertook an in-depth analysis of edited RNA populations in TbRGG2 knockdown cells and an in vitro examination of the biochemical activities of the protein. We demonstrate that TbRGG2 down-regulation more severely impacts editing at the 5' ends of pan-edited RNAs than at their 3' ends. The initiation of editing is reduced to some extent in TbRGG2 knockdown cells. In addition, TbRGG2 plays a post-initiation role as editing becomes stalled in TbRGG2-depleted cells, resulting in an overall decrease in the 3' to 5' progression of editing. Detailed analyses of edited RNAs from wild-type and TbRGG2-depleted cells reveal that TbRGG2 facilitates progression of editing past intrinsic pause sites that often correspond to the 3' ends of cognate guide RNAs (gRNAs). In addition, noncanonically edited junction regions are either absent or significantly shortened in TbRGG2-depleted cells, consistent with impaired gRNA transitions. Sequence analysis further suggests that TbRGG2 facilitates complete utilization of certain gRNAs. In vitro RNA annealing and in vivo RNA unwinding assays demonstrate that TbRGG2 can modulate RNA-RNA interactions. Collectively, these data are consistent with a model in which TbRGG2 facilitates initiation and 3' to 5' progression of editing through its ability to affect gRNA utilization, both during the transition between specific gRNAs and during usage of certain gRNAs.
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