ArticleAustralian veterinary journal2010
Application of high-resolution melt curve analysis for classification of infectious bronchitis viruses in field specimens.
Article in Australian veterinary journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Development of an HRM-qRT-PCR platform for fast and cost-effective genotyping of infectious bronchitis virus in Egypt.Scientific reports · 2026Article
- Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting.PloS one · 2021Article
- High-resolution melting curve analysis for infectious bronchitis virus strain differentiation.Veterinary world · 2020Article
- A Novel Immunochromatographic Strip for Antigen Detection of Avian Infectious Bronchitis Virus.International journal of molecular sciences · 2019Article
- Detection and Differentiation of Two Koala Gammaherpesviruses by Use of High-Resolution Melt (HRM) Analysis Reveals Differences in Viral Prevalence and Clinical Associations in a Large Study of Free-Ranging Koalas.Journal of clinical microbiology · 2019Article
- Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis.Advances in virology · 2016Review
- Assessment of the potential relationship between egg quality and infectious bronchitis virus infection in Australian layer flocks.Australian veterinary journal · 2014Article
- Article
- Real-time PCR and high-resolution melt analysis for rapid detection of Mycobacterium leprae drug resistance mutations and strain types.Journal of clinical microbiology · 2012Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveA real-time polymerase chain reaction (PCR)/high-resolution melt (HRM) curve analysis protocol was developed in our laboratory to differentiate infectious bronchitis (IB) virus reference strains. In the current study, this method was used to detect and classify IB viruses in field submissions. PROCEDURE: Over an 11-month period samples from 40 cases of suspected IB virus were received and 17 submissions were positive for IB virus by polymerase chain reaction. HRM curve analysis classified each strain as subgroup 1, 2 or 3 strain (12 submissions) or a strain that was unable to be classified (5 submissions). The 3' untranslated region (UTR) and partial S1 gene nucleotide sequences for the 17 IB virus strains were determined and their identity with those of the relative reference strains compared to confirm the classifications generated using the HRM curve analysis.
resultsOf the 12 IB field viruses classified as subgroup 1, 2, or 3 using HRM curve analysis, the 3'UTR and S1 gene nucleotide sequences had identities ≥99% with the respective subgroup reference strain. Analysis of the 3' UTR and S1 gene nucleotide sequences for the five IB virus strains that could not be classified indicated that four belonged to one of the subgroups, and one was a potential recombinant strain (between strains from subgroups 2 and 3). A novel recombinant strain was also detected.
conclusionHRM curve analysis can rapidly assign the majority of IB viruses present in field submissions to known subgroups. Importantly, HRM curve analysis also identified variant genotypes that require further investigation.
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