ArticleBiochemistry2010
The ATP-dependent remodeler RSC transfers histone dimers and octamers through the rapid formation of an unstable encounter intermediate.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- ATP-dependent remodeling of chromatin condensates reveals distinct mesoscale outcomes.Science (New York, N.Y.) · 2025Article
- Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF.Science advances · 2022Article
- Local chromatin context regulates the genetic requirements of the heterochromatin spreading reaction.PLoS genetics · 2022Article
- SMARCAD1 is an ATP-dependent histone octamer exchange factor with de novo nucleosome assembly activity.Science advances · 2021Article
- Aurora kinase A, a synthetic lethal target for precision cancer medicine.Experimental & molecular medicine · 2021Review
- Interaction of RSC Chromatin Remodeling Complex with Nucleosomes Is Modulated by H3 K14 Acetylation and H2B SUMOylation In Vivo.iScience · 2020Article
- Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.eLife · 2019Article
- Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes.Nature reviews. Molecular cell biology · 2017Review
- Response to Comment on "A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme".Science (New York, N.Y.) · 2016Article
- Mechanisms of ATP-Dependent Chromatin Remodeling Motors.Annual review of biophysics · 2016Review
- Article
- Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus.Molecular and cellular biology · 2016Article
- Article
- Preparation of mononucleosomal templates for analysis of transcription with RNA polymerase using spFRET.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Preparation and analysis of positioned mononucleosomes.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Histone H3 phosphorylation near the nucleosome dyad alters chromatin structure.Nucleic acids research · 2014Article
- Single-molecule tools elucidate H2A.Z nucleosome composition.Journal of cell science · 2012Article
- Swi2/Snf2 remodelers: hybrid views on hybrid molecular machines.Current opinion in structural biology · 2012Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RSC, an essential chromatin remodeling complex in budding yeast, is involved in a variety of biological processes including transcription, recombination, repair, and replication. How RSC participates in such diverse processes is not fully understood. In vitro, RSC uses ATP to carry out several seemingly distinct reactions: it repositions nucleosomes, transfers H2A/H2B dimers between nucleosomes, and transfers histone octamers between pieces of DNA. This raises the intriguing mechanistic question of how this molecular machine can use a single ATPase subunit to create these varied products. Here, we use a FRET-based approach to kinetically order the products of the RSC reaction. Surprisingly, transfer of H2A/H2B dimers and histone octamers is initiated on a time scale of seconds when assayed by FRET, but formation of stable nucleosomal products occurs on a time scale of minutes when assayed by native gel. These results suggest a model in which RSC action rapidly generates an unstable encounter intermediate that contains the two exchange substrates in close proximity. This intermediate then collapses more slowly to form the stable transfer products seen on native gels. The rapid, biologically relevant time scale on which the transfer products are generated implies that such products can play key roles in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.