ArticlePlant cell reports2010
Mature seed-derived callus of the model indica rice variety Kasalath is highly competent in Agrobacterium-mediated transformation.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- An improved Agrobacterium-mediated transformation method for genome editing using CRISPR/Cas9 in elite indica rice (Oryza sativa L.).BMC genomics · 2026Article
- Article
- Agrobacterium-Mediated Transformation and Targeted Mutagenesis Using SpCas12f in Rice.Methods in molecular biology (Clifton, N.J.) · 2025Article
- An Efficient Transformation System for Fast Production ofPlants (Basel, Switzerland) · 2023Article
- QTLs Related to Rice Callus Regeneration Ability: Localization and Effect Verification ofCells · 2022Article
- An EfficientInternational journal of molecular sciences · 2022Article
- Article
- Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (International journal of molecular sciences · 2021Article
- Article
- Characterization and Evaluation of Transgenic Rice Pyramided with the Pi Genes Pib, Pi25 and Pi54.Rice (New York, N.Y.) · 2021Article
- Natural variations at the Stay-Green gene promoter control lifespan and yield in rice cultivars.Nature communications · 2020Article
- Robust and ReproducibleFrontiers in plant science · 2020Article
- A Universal System of CRISPR/Cas9-Mediated Gene Targeting Using All-in-One Vector in Plants.Frontiers in genome editing · 2020Article
- Cloning, transformation and expression of cell cycle-associated protein kinaseJournal, genetic engineering & biotechnology · 2018Article
- Progress in Research and Application of HIV-1 TAT-Derived Cell-Penetrating Peptide.The Journal of membrane biology · 2017Review
- A Defect in DNA Ligase4 Enhances the Frequency of TALEN-Mediated Targeted Mutagenesis in Rice.Plant physiology · 2016Article
- Precision genome editing in plants via gene targeting and piggyBac-mediated marker excision.The Plant journal : for cell and molecular biology · 2015Article
- Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat.Protoplasma · 2014Article
- Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation.Protoplasma · 2014Article
- The non-homologous end-joining pathway is involved in stable transformation in rice.Frontiers in plant science · 2014Review
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2 authors.
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Abstract
We previously established an efficient Agrobacterium-mediated transformation system using primary calli derived from mature seeds of the model japonica rice variety Nipponbare. We expected that the shortened tissue culture period would reduce callus browning--a common problem with the indica transformation system during prolonged tissue culture in the undifferentiated state. In this study, we successfully applied our efficient transformation system to Kasalath--a model variety of indica rice. The Luc reporter system is sensitive enough to allow quantitative analysis of the competency of rice callus for Agrobacterium-mediated transformation. We unexpectedly discovered that primary callus of Kasalath exhibits a remarkably high competency for Agrobacterium-mediated transformation compared to Nipponbare. Southern blot analysis and Luc luminescence showed that independent transformation events in primary callus of Kasalath occurred successfully at ca. tenfold higher frequency than in Nipponbare, and single copy T-DNA integration was observed in ~40% of these events. We also compared the competency of secondary callus of Nipponbare and Kasalath and again found superior competency in Kasalath, although the identification and subsequent observation of independent transformation events in secondary callus is difficult due to the vigorous growth of both transformed and non-transformed cells. An efficient transformation system in Kasalath could facilitate the identification of QTL genes, since many QTL genes are analyzed in a Nipponbare × Kasalath genetic background. The higher transformation competency of Kasalath could be a useful trait in the establishment of highly efficient systems involving new transformation technologies such as gene targeting.
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