ArticleMolecular genetics and genomics : MGG2010
Development of an efficient gene targeting system in Colletotrichum higginsianum using a non-homologous end-joining mutant and Agrobacterium tumefaciens-mediated gene transfer.
Article in Molecular genetics and genomics : MGG, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 32 citations in OpenAlex.
- Confrontations betweenApplied and environmental microbiology · 2026Article
- Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/Cas9 ribonucleoprotein and URA3-based marker recycling.Molecular plant pathology · 2023Article
- Establishment of a selection marker recycling system for sequential transformation of the plant-pathogenic fungus Colletotrichum orbiculare.Molecular plant pathology · 2019Article
- Review
- ChMob2 binds to ChCbk1 and promotes virulence and conidiation of the fungal pathogen Colletotrichum higginsianum.BMC microbiology · 2017Article
- Biallelic Gene Targeting in Rice.Plant physiology · 2016Article
- Colletotrichum higginsianum Mitogen-Activated Protein Kinase ChMK1: Role in Growth, Cell Wall Integrity, Colony Melanization, and Pathogenicity.Frontiers in microbiology · 2016Article
- A ku70 null mutant improves gene targeting frequency in the fungal pathogen Verticillium dahliae.World journal of microbiology & biotechnology · 2015Article
- Article
- Improved Gene Targeting through Cell Cycle Synchronization.PloS one · 2015Article
- The Colletotrichum destructivum species complex - hemibiotrophic pathogens of forage and field crops.Studies in mycology · 2014Article
- Bidirectional-genetics platform, a dual-purpose mutagenesis strategy for filamentous fungi.Eukaryotic cell · 2013Article
- Characterization of a fungal thioesterase having Claisen cyclase and deacetylase activities in melanin biosynthesis.Chemistry & biology · 2012Article
- Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice.The New phytologist · 2012Article
- Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses.Nature genetics · 2012Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hemibiotrophic ascomycete Colletotrichum higginsianum is the casual agent of anthracnose disease of cruciferous plants. High efficiency transformation by Agrobacterium tumefaciens-mediated gene transfer has been established for this fungus. However, targeted gene mutagenesis through homologous recombination rarely occurs in C. higginsianum. We have identified and disrupted the C. higginsianum homologue of the human Ku70 gene, ChKU70, which encodes a protein that plays a role in non-homologous end-joining for repair of DNA breaks. chku70 mutants showed a dramatic increase in the frequency of integration of introduced exogenous DNA fragments by homologous recombination without any detectable phenotypic defects. This result demonstrates that the chku70 mutant is an efficient recipient for targeted gene mutagenesis in C. higginsianum. We have also developed a novel approach [named direct repeat recombination-mediated gene targeting (DRGT)] for targeted gene disruption through Agrobacterium tumefaciens-mediated gene transfer. DRGT utilizes homologous recombination between repeated sequences on the T-DNA flanking a partial fragment of the target gene. Our results suggest that DRGT in the chku70 mutant background could be a useful tool for rapid isolation of targeted gene disruptants in C. higginsianum.
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