ArticleParasitology research2011
Screening the Schistosoma mansoni transcriptome for genes differentially expressed in the schistosomulum stage in search for vaccine candidates.
Article in Parasitology research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 48 citations in OpenAlex.
- In Search of the Role of Three-Finger Starfish Proteins.Marine drugs · 2024Article
- Anti-schistosomal immunity to core xylose/fucose in N-glycans.Frontiers in molecular biosciences · 2023Article
- Screening of a Library of Recombinant Schistosoma mansoni Proteins With Sera From Murine and Human Controlled Infections Identifies Early Serological Markers.The Journal of infectious diseases · 2022Article
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- Optimization of Expression and Purification of Schistosoma mansoni Antigens in Fusion with Rhizavidin.Molecular biotechnology · 2021Article
- Assessment of reference genes at six different developmental stages of Schistosoma mansoni for quantitative RT-PCR.Scientific reports · 2021Article
- Immune Evasion Strategies of Schistosomes.Frontiers in immunology · 2020Review
- Schistosoma mansoni venom allergen-like proteins: phylogenetic relationships, stage-specific transcription and tissue localization as predictors of immunological cross-reactivity.International journal for parasitology · 2019Article
- In vitro activity of aryl-thiazole derivatives against Schistosoma mansoni schistosomula and adult worms.PloS one · 2019Article
- Systematic screening of 96Wellcome open research · 2019Article
- Antibody responses to Schistosoma mansoni schistosomula antigens.Parasite immunology · 2018Article
- Schistosoma mansoni schistosomula antigens induce Th1/Pro-inflammatory cytokine responses.Parasite immunology · 2018Article
- Stage and tissue expression patterns of Schistosoma mansoni venom allergen-like proteins SmVAL 4, 13, 16 and 24.Parasites & vectors · 2017Article
- Human IgG1 Responses to Surface Localised Schistosoma mansoni Ly6 Family Members Drop following Praziquantel Treatment.PLoS neglected tropical diseases · 2015Article
- Discovery of novel Schistosoma japonicum antigens using a targeted protein microarray approach.Parasites & vectors · 2014Article
- Interaction Between the Neglected Tropical Disease Human Schistosomiasis and HCV Infection in Egypt: a Puzzling Relationship.Journal of clinical and translational hepatology · 2014Review
- Comparative evaluation of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium alkaline phosphatase antigenicity by the alkaline phosphatase immunoassay (APIA).Parasitology research · 2014Article
- Transcriptome profilings of female Schistosoma japonicum reveal significant differential expression of genes after pairing.Parasitology research · 2014Article
- Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host.Parasitology research · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Schistosomiasis affects more than 200 million people worldwide; another 600 million are at risk of infection. The schistosomulum stage is believed to be the target of protective immunity in the attenuated cercaria vaccine model. In an attempt to identify genes up-regulated in the schistosomulum stage in relation to cercaria, we explored the Schistosoma mansoni transcriptome by looking at the relative frequency of reads in EST libraries from both stages. The 400 genes potentially up-regulated in schistosomula were analyzed as to their Gene Ontology categorization, and we have focused on those encoding-predicted proteins with no similarity to proteins of other organisms, assuming they could be parasite-specific proteins important for survival in the host. Up-regulation in schistosomulum relative to cercaria was validated with real-time reverse transcription polymerase chain reaction (RT-PCR) for five out of nine selected genes (56%). We tested their protective potential in mice through immunization with DNA vaccines followed by a parasite challenge. Worm burden reductions of 16-17% were observed for one of them, indicating its protective potential. Our results demonstrate the value and caveats of using stage-associated frequency of ESTs as an indication of differential expression coupled to DNA vaccine screening in the identification of novel proteins to be further investigated as potential vaccine candidates.
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