ArticleCellular and molecular bioengineering2008
Fluid Flow Induced Calcium Response in Bone Cell Network.
Article in Cellular and molecular bioengineering, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The trial behind it
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Who cites it
28 citing papers in PubMed, 66 citations in OpenAlex.
- Functional Roles of Connexins and Gap Junctions in Osteo-Chondral Cellular Components.International journal of molecular sciences · 2023Review
- Symmetry breaking and effects of nutrient walkway in time-dependent bone remodeling incorporating poroelasticity.Biomechanics and modeling in mechanobiology · 2022Article
- Physical and Chemical Characterization of Biomineralized Collagen with Different Microstructures.Journal of functional biomaterials · 2022Article
- Piezo1 channel activation in response to mechanobiological acoustic radiation force in osteoblastic cells.Bone research · 2021Article
- Molecular mechanosensors in osteocytes.Bone research · 2020Review
- Spreading Shape and Area Regulate the Osteogenesis of Mesenchymal Stem Cells.Tissue engineering and regenerative medicine · 2019Article
- Calcium response in bone cells at different osteogenic stages under unidirectional or oscillatory flow.Biomicrofluidics · 2019Article
- Spontaneous calcium signaling of cartilage cells: from spatiotemporal features to biophysical modeling.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Bioprinting and Organ-on-Chip Applications Towards Personalized Medicine for Bone Diseases.Stem cell reviews and reports · 2017Review
- The molecular basis of bone mechanotransduction.Journal of musculoskeletal & neuronal interactions · 2016Review
- Effects of Osmolarity on the Spontaneous Calcium Signaling of In Situ Juvenile and Adult Articular Chondrocytes.Annals of biomedical engineering · 2016Article
- Experimental studies of bone mechanoadaptation: bridging in vitro and in vivo studies with multiscale systems.Interface focus · 2016Article
- Connexins in the skeleton.Seminars in cell & developmental biology · 2016Review
- Article
- The effect of chemically defined medium on spontaneous calcium signaling of in situ chondrocytes during long-term culture.Journal of biomechanics · 2015Article
- Integrative transcriptomic and proteomic analysis of osteocytic cells exposed to fluid flow reveals novel mechano-sensitive signaling pathways.Journal of biomechanics · 2014Article
- Purinergic signalling in the musculoskeletal system.Purinergic signalling · 2013Review
- Article
- Mechanically induced intercellular calcium communication in confined endothelial structures.Biomaterials · 2013Article
- Involvement of large conductance Ca(2+)-activated K (+) channel in laminar shear stress-induced inhibition of vascular smooth muscle cell proliferation.Pflugers Archiv : European journal of physiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
In our previous work, bone cell networks with controlled spacing and functional intercellular gap junctions had been successfully established by using microcontact printing and self assembled monolayers technologies [Guo, X. E., E. Takai, X. Jiang, Q. Xu, G. M. Whitesides, J. T. Yardley, C. T. Hung, E. M. Chow, T. Hantschel, and K. D. Costa. Mol. Cell. Biomech. 3:95-107, 2006]. The present study investigated the calcium response and the underlying signaling pathways in patterned bone cell networks exposed to a steady fluid flow. The glass slides with cell networks were separated into eight groups for treatment with specific pharmacological agents that inhibit pathways significant in bone cell calcium signaling. The calcium transients of the network were recorded and quantitatively evaluated with a set of network parameters. The results showed that 18α-GA (gap junction blocker), suramin (ATP inhibitor), and thapsigargin (depleting intracellular calcium stores) significantly reduced the occurrence of multiple calcium peaks, which were visually obvious in the untreated group. The number of responsive peaks also decreased slightly yet significantly when either the COX-2/PGE(2) or the NOS/nitric oxide pathway was disrupted. Different from all other groups, cells treated with 18α-GA maintained a high concentration of intracellular calcium following the first peak. In the absence of calcium in the culture medium, the intracellular calcium concentration decreased slowly with fluid flow without any calcium transients observed. These findings have identified important factors in the flow mediated calcium signaling of bone cells within a patterned network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.