ArticleProceedings of the National Academy of Sciences of the United States of America2010
Absence of branches from xylan in Arabidopsis gux mutants reveals potential for simplification of lignocellulosic biomass.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 143 papers.
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Who cites it
143 citing papers in PubMed, 339 citations in OpenAlex.
- Mass distribution profiling of cell wall matrix polysaccharide biosynthesis mutants reveals alterations in non-targeted matrix polysaccharides.Cell surface (Amsterdam, Netherlands) · 2026Article
- Comparison of synthetic and post-synthetic molecular genetic methods of reducing xylan glucuronidation in hybrid aspen for biorefinery applications.Biotechnology for biofuels and bioproducts · 2026Article
- Dissecting genetic architecture and improving machine learning‑based genomic prediction of flowering time in Osmanthus fragrans by integrating structural variants.The plant genome · 2026Article
- Origins of Noncovalent Interactions in Lignocellulosic Biomass and Potential Implications on Recalcitrance.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Fine-tuning xylan side chains for enhanced production of high-yield sugars, fine-shaped nanocrystals, and stable Pickering emulsions in Arabidopsis thaliana.Biotechnology for biofuels and bioproducts · 2026Article
- Wood-specific modification of glucuronoxylan can enhance growth in Populus.Journal of experimental botany · 2026Article
- Glucuronoyl Esterase Expressed in Aspen Xylem Affects γ-Ester Linkages Between Lignin and Glucuronoxylan Reducing Recalcitrance and Accelerating Growth.Plant biotechnology journal · 2025Article
- GT61 β-1,2-xylosyltransferases define a conserved xylan modification in gymnosperm and Arabidopsis primary cell walls.The Plant journal : for cell and molecular biology · 2025Article
- Physiological and molecular mechanisms of nitrogen in alleviating drought stress in Phoebe bournei.Scientific reports · 2025Article
- Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets.Journal of the American Chemical Society · 2025Article
- A Fresh Look at Celery Collenchyma and Parenchyma Cell Walls Through a Combination of Biochemical, Histochemical, and Transcriptomic Analyses.International journal of molecular sciences · 2025Article
- Identification of the RNA m5C methyltransferase genes inForestry research · 2025Article
- XYLAN O-ACETYLTRANSFERASE 6 promotes xylan synthesis by forming a complex with IRX10 and governs wall formation in rice.The Plant cell · 2024Article
- Identification of glycosyltransferases mediating 2-O-arabinopyranosyl and 2-O-galactosyl substitutions of glucuronosyl side chains of xylan.The Plant journal : for cell and molecular biology · 2024Article
- In Vitro GT-array (Bio-protocol · 2024Article
- Plant Cell Wall PolysaccharidePlants (Basel, Switzerland) · 2024Review
- Genome-Wide Identification and Functional Analysis of the GUX Gene Family inInternational journal of molecular sciences · 2024Article
- Photoperiod-1 regulates the wheat inflorescence transcriptome to influence spikelet architecture and flowering time.Current biology : CB · 2024Article
- Modifying lignin composition and xylan O-acetylation induces changes in cell wall composition, extractability, and digestibility.Biotechnology for biofuels and bioproducts · 2024Article
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
83 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
As one of the most abundant polysaccharides on Earth, xylan will provide more than a third of the sugars for lignocellulosic biofuel production when using grass or hardwood feedstocks. Xylan is characterized by a linear β(1,4)-linked backbone of xylosyl residues substituted by glucuronic acid, 4-O-methylglucuronic acid or arabinose, depending on plant species and cell types. The biological role of these decorations is unclear, but they have a major influence on the properties of the polysaccharide. Despite the recent isolation of several mutants with reduced backbone, the mechanisms of xylan synthesis and substitution are unclear. We identified two Golgi-localized putative glycosyltransferases, GlucUronic acid substitution of Xylan (GUX)-1 and GUX2 that are required for the addition of both glucuronic acid and 4-O-methylglucuronic acid branches to xylan in Arabidopsis stem cell walls. The gux1 gux2 double mutants show loss of xylan glucuronyltransferase activity and lack almost all detectable xylan substitution. Unexpectedly, they show no change in xylan backbone quantity, indicating that backbone synthesis and substitution can be uncoupled. Although the stems are weakened, the xylem vessels are not collapsed, and the plants grow to normal size. The xylan in these plants shows improved extractability from the cell wall, is composed of a single monosaccharide, and requires fewer enzymes for complete hydrolysis. These findings have implications for our understanding of the synthesis and function of xylan in plants. The results also demonstrate the potential for manipulating and simplifying the structure of xylan to improve the properties of lignocellulose for bioenergy and other uses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.