ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2010
Site-specific differences in gene expression of secreted proteins in the mouse lung: comparison of methods to show differences by location.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Impact of aging and ergothioneine pre-treatment on naphthalene toxicity in lung.Toxicology letters · 2024Article
- Metabolism and Lung Toxicity of Inhaled Naphthalene: Effects of Postnatal Age and Sex.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- Characterization of the cell of origin and propagation potential of the fibroblast growth factor 9-induced mouse model of lung adenocarcinoma.The Journal of pathology · 2015Article
- Ozone-induced airway epithelial cell death, the neurokinin-1 receptor pathway, and the postnatal developing lung.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Ozone exposure alters serotonin and serotonin receptor expression in the developing lung.Toxicological sciences : an official journal of the Society of Toxicology · 2013Article
- Novel lanthanide-labeled metal oxide nanoparticles improve the measurement of in vivo clearance and translocation.Particle and fibre toxicology · 2013Article
- Postnatal exposure history and airways: oxidant stress responses in airway explants.American journal of respiratory cell and molecular biology · 2012Article
- Sex differences in the development of airway epithelial tolerance to naphthalene.American journal of physiology. Lung cellular and molecular physiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Studies on the effects of pulmonary toxicants on the lung often overlook the fact that site-specific changes are likely to occur in response to chemical exposure. These changes can be highly focal and may be undetected by methods that do not examine specific lung regions. This problem is especially acute for studies of the conducting airways. In this study, differential gene expression of secreted proteins in the lung by different methods of collection (whole lung, gross airway microdissection, and laser capture microdissection) and by airway levels (whole lobe, whole airway tree, proximal airways, airway bifurcations, and terminal bronchioles) was examined. Site-specific sampling approaches were combined with methods to detect both gene and corresponding protein expression in different lung regions. Differential expression of mRNA by both airway level and lung region was determined for Clara cell secretory protein, calcitonin gene-related peptide, uteroglobin-related protein 2, surfactant protein A, and surfactant protein C. Therefore, for maximal enrichment of mRNA and maximal ability to identify changes in mRNA levels in the diseased state or in response to chemical exposure, it is critical to choose the appropriate airway region and sample collection method to enrich detection of the transcript(s) of interest.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.