Evidence map›Paper›PMID 20851986›Full record

ArticleApplied and environmental microbiology2010

Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.

Nicolas Radomski, Françoise S Lucas, Régis Moilleron, Emmanuelle Cambau, Sophie Haenn, Laurent Moulin

Abstract readComparative Study
In one paragraph

Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Discovery and Characterization ofFrontiers in microbiology · 2018
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nicolas RadomskiUniversité Paris-Est, Laboratoire Eau Environnement Systèmes Urbains UMR MA 102-AgroParisTech, 6-8 avenue Blaise Pascal Cité Descartes, FR 77455 Champs sur Marne, France. nicolas.radomski@leesu.enpc.fr
Françoise S Lucas
Régis Moilleron
Emmanuelle Cambau
Sophie Haenn
Laurent Moulin

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).

Indexed as

Water MicrobiologyBacterial LoadDNA, BacterialGenes, BacterialMolecular Sequence DataMycobacteriumPolymerase Chain ReactionRNA, Ribosomal, 16SSensitivity and SpecificityDNA, BacterialRNA, Ribosomal, 16S

Identifiers

PMID20851986
PMCPMC2976254

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.