Evidence map›Paper›PMID 20851971›Full record

ArticleApplied and environmental microbiology2010

Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece.

Hongtao Min, Louis A Sherman

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Review
  3. Enhanced Nitrogen Fixation in aApplied and environmental microbiology · 2019
    Article
  4. Article
  5. Article
  6. Transcriptional regulators ChlR and CnfR are essential for diazotrophic growth in nonheterocystous cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2014
    Article
  7. Article
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Hongtao MinPurdue University, Department of Biological Sciences, West Lafayette, IN 47907, USA.
Louis A Sherman
Purdue University West Lafayette · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a genetic system for producing specific gene knockouts in Cyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen.

Indexed as

MutagenesisTransformation, GeneticCulture MediaCyanotheceDNA, Single-StrandedGene Knockout TechniquesGenes, BacterialGenetics, MicrobialHydrogenCulture MediaDNA, Single-StrandedHydrogen

Identifiers

PMID20851971
PMCPMC2976206
OpenAlexW2109399911

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.